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Index / MarkerGene / MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che / Product Detail : M0855 MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che
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#M0855 MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che

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  Price : 422   EUR
479   USD
327   GBP
1775   Zloty
56540   JPY
3258   NOK
3491   SEK
477   CHF

Product name : MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che

Catalog number : M0855

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Availability: Yes

Supplier name : MarkerGene

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About this Product :

MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of MarkerGeneTM Chemiluminescent lacZ beta_Galactosidase Detection Kit, Allows for quantitaive measure of b_galactosidase enzyme activity in mammalian, yeast, and bacterial cells through the use of a che.
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Description:
Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate.

Application:
One of the most common reporter genes used in molecular biology applications is the E. coli lacZ gene that codes for an active subunit of β-galactosidase in vivo. Since this enzyme is generally absent in normal mammalian, yeast, some bacterial and even plant cells, it can be detected at very low levels, and due to its wide substrate specificity, monitoring lacZ expression (and therefore co-expressed genes or promoter efficiency) has become routine to the point of detection of as few as 5 copies of β-galactosidase. Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) are useful, the recent application of chemiluminescent 1,2 Dioxetane substrates, which emit visible light upon enzyme catalysis, provide rapid results with very low background and high intensity signal. An enhancing solution is also provided with this kit to increase light production efficiency in solution-based assays by drawing water away from the signal production site. The Chemiluminescent lacZ β-Galactosidase Detection Kit provides all the necessary reagents, buffers, substrate, and protocols for sensitive and quantitative lacZ β-galactosidase activity assay. Please contact our technical services department for further information.

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MarkerGene \ MarkerGeneTM_Chemiluminescent_lacZ_beta_Galactosidase_Detection_Kit,_Allows_for_quantitaive_measure_of_b_galactosidase_enzyme_activity_in_mammalian,_yeast,_and_bacterial_cells_through_the_use_of_a_che \ M0855
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