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Index / MarkerGene / pCMVbeta Mammalian lacZco Expression Vector, 20micrograms / Product Detail : M1015 pCMVbeta Mammalian lacZco Expression Vector, 20micrograms
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Sep
28th
#M1015 pCMVbeta Mammalian lacZco Expression Vector, 20micrograms
| Price : | 451 | EUR |
| 512 | USD | |
| 350 | GBP | |
| 1896 | Zloty | |
| 60405 | JPY | |
| 3481 | NOK | |
| 3730 | SEK | |
| 510 | CHF |
Product name : pCMVbeta Mammalian lacZco Expression Vector, 20micrograms
Catalog number : M1015
Quantity:
Availability: Yes
Supplier name : MarkerGene
Data sheet: Ask more or other datasheet now !
More Details about
Description:
High copy number eukaryotic vector, pCMVβ expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of the cytomegalovirus immediate early gene (CMV IE) promoter.
Application:
When expressed in mammalian cells, the codon-optimized gene results in expression levels of β-galactosidase 15-fold higher than those resulting from an analogous construct containing the native E. coli gene sequence. Enhanced transcript stability and increased translational efficiency provide for increased β-galactosidase expression, as suggested by RNA analysis. In addition, codon-optimization results in the elimination of several cryptic splice acceptor sites that are present in the native E. coli gene sequence and increases the amounts of un-spliced, full-length genomic RNA when used in a lentiviral vector containing a 5' splice donor. The β-galactosidase enzyme expression is also enhanced by the SV40 late polyadenylylation signal. This pCMVβ expression vector contains the β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. pCMVβ vector has been tested to generate up to 2530u/mg cell extract (MacGregor, and Caskey). If desired, the β-galactosidase codon optimized gene can be excised using XhoI and NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our pSV40β lacZco vector system
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Related Genes :
[] Non-structural polyprotein (Nonstructural polyprotein)
[] Structural polyprotein
Bibliography :
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[19402626] Guanidinylated polyethyleneimine-polyoxypropylene-polyoxyethylene conjugates as gene transfection agents.
[19332382] Cationic liposome-DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells.
[19010873] Novel jet-injection technology for nonviral intratumoral gene transfer in patients with melanoma and breast cancer.
[18830969] Gene delivery into hepatocytes with the preS/liposome/DNA system.
[17671832] Gene delivery to the epidermal cells of human skin explants using microfabricated microneedles and hydrogel formulations.
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