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Index / MarkerGene / pSV40beta Mammalian lacZnls12co Expression Vector, 20micrograms / Product Detail : M1018 pSV40beta Mammalian lacZnls12co Expression Vector, 20micrograms
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#M1018 pSV40beta Mammalian lacZnls12co Expression Vector, 20micrograms

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  Price : 470   EUR
534   USD
365   GBP
1977   Zloty
62981   JPY
3630   NOK
3889   SEK
532   CHF

Product name : pSV40beta Mammalian lacZnls12co Expression Vector, 20micrograms

Catalog number : M1018

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Availability: Yes

Supplier name : MarkerGene

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Description: 
Eukaryotic expression vector, pSV40β expresses the full-length codon-optimized β-galactosidase gene (lacZco) under the control of simian virus 40 (SV40) early promoter.

Application: 
When expressed in mammalian cells, the codon-optimized gene results in expression levels of β-galactosidase 15-fold higher than those resulting from an analogous construct containing the native E. coli gene sequence. Enhanced transcript stability and increased translational efficiency provide for increased β-galactosidase expression, as suggested by RNA analysis. In addition, codon-optimization results in the elimination of several cryptic splice acceptor sites that are present in the native E. coli gene sequence and increases the amounts of un-spliced, full-length genomic RNA when used in a lentiviral vector containing a 5'-splice donor. The β-galactosidase enzyme expression is also enhanced by the SV40 late polyadenylylation signal. The pSV40β expression vector also contains β-lactamase, which acts as a selection marker (100 μg/ml ampicillin resistance) in E. coli host. If desired, the β-galactosidase codon optimized gene can be excised using XhoI and NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells.

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