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Index / MarkerGene / Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg / Product Detail : M1166 Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg
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#M1166 Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg

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  Price : 232   EUR
263   USD
180   GBP
975   Zloty
31061   JPY
1790   NOK
1918   SEK
262   CHF

Product name : Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg

Catalog number : M1166

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Availability: Yes

Supplier name : MarkerGene

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More Details about

Description:
One of the most popular substrates for analysis of microsomal dealkylase and cytochrome P450 enzymes.

Application:
It produces the red fluorescent dye resorufin (EX/EM 571/585 nm) upon enzyme activity. This substrate has also been extensively used to differentiate the various isozymes of the Cytochrome P450 system.

Note:
The cytochome P450's are a large group of monooxygenase enzymes responsible for the metabolism of toxins in the body. These enzymes, requiring NADPH as a cofactor and O2 as co-substrate, are located in the endoplasmic reticulum and are highly concentrated in the liver and small intestine. Additionally, Cytochrome P450's are also found in the mitochondrial membrane. P450 enzymes encompass a highly diverse "superfamily" of hemoproteins, and metabolic oxidation of chemical compounds is the main function of the cytochrome-mediated monooxygenase or mixed-function oxidase system. Cytochrome P450 is also used as a marker for the endoplasmic reticulum. The active site of cytochrome P450 contains the catalyst heme (iron-porphyrin) with a sulfur of cysteine serving as the fifth iron ligand. Although these enzymes typically have a low turnover rate, their intracellular activity can be monitored using fluorogenic alkyl ether derivatives of fluorophores including resorufin. Ex = 463 nm [EX/EM 571/585 nm of resorufin enzymatic product]

Molecular Weight: 227.22

Storage: C, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: DMSO

Molecular Formula: C13H9O3N

M1166

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