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Index / MarkerGene / Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg / Product Detail : M1166 Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg
#M1166 Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg
Product name : Resorufin 7_O_methyl ether, For analysis of microsomal dealkylase and cytochrome P450 enzymes, 5mg
Catalog number : M1166
Supplier name : MarkerGene
Data sheet: Ask more or other datasheet now !
More Details about
One of the most popular substrates for analysis of microsomal dealkylase and cytochrome P450 enzymes.
It produces the red fluorescent dye resorufin (EX/EM 571/585 nm) upon enzyme activity. This substrate has also been extensively used to differentiate the various isozymes of the Cytochrome P450 system.
The cytochome P450's are a large group of monooxygenase enzymes responsible for the metabolism of toxins in the body. These enzymes, requiring NADPH as a cofactor and O2 as co-substrate, are located in the endoplasmic reticulum and are highly concentrated in the liver and small intestine. Additionally, Cytochrome P450's are also found in the mitochondrial membrane. P450 enzymes encompass a highly diverse "superfamily" of hemoproteins, and metabolic oxidation of chemical compounds is the main function of the cytochrome-mediated monooxygenase or mixed-function oxidase system. Cytochrome P450 is also used as a marker for the endoplasmic reticulum. The active site of cytochrome P450 contains the catalyst heme (iron-porphyrin) with a sulfur of cysteine serving as the fifth iron ligand. Although these enzymes typically have a low turnover rate, their intracellular activity can be monitored using fluorogenic alkyl ether derivatives of fluorophores including resorufin. Ex = 463 nm [EX/EM 571/585 nm of resorufin enzymatic product]
Molecular Weight: 227.22
Storage: C, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Molecular Formula: C13H9O3N
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WP1077: cytochrome P450
WP1194: cytochrome P450
WP1219: cytochrome P450
WP1274: cytochrome P450
WP1390: cytochrome P450
WP2316: PPAR signaling pathway
WP43: cytochrome P450
WP677: Momilactone biosynthesis
WP696: Benzo(a)pyrene metabolism
WP958: cytochrome P450
WP1021: Phase I, non P450
WP1140: Phase I, non P450
WP1213: Phase I, non P450
WP1255: Phase I, non P450
WP1291: Phase I, non P450
WP136: Phase I, non P450
WP1502: Mitochondrial biogenesis
WP1573: Phase I, non P450
WP1645: Ether lipid metabolism
WP1963: The effect of Glucocorticoids on target gene expression
WP2430: Quercetin metabolism
WP668: Octadecanoid Pathway
WP791: Phase I, non P450
WP904: Phase I, non P450
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