GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, firstname.lastname@example.org
Index / MarkerGene / MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect â_galactosidase activity in senescent cells at pH 6.0 using X_Gal, 1 kit / Product Detail : M1389 MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect â_galactosidase activity in senescent cells at pH 6.0 using X_Gal, 1 kit
#M1389 MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect â_galactosidase activity in senescent cells at pH 6.0 using X_Gal, 1 kit
Product name : MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect â_galactosidase activity in senescent cells at pH 6.0 using X_Gal, 1 kit
Catalog number : M1389
Supplier name : MarkerGene
Data sheet: Ask more or other datasheet now !
More Details about
Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal.
Many primary cell types, particularly fibroblast cells, have a limited capacity to reproduce in cell culture. Even normal cells, derived from fetal, embryonic or newborn tissue, typically undergo between 40 and 60 cell divisions, but then often stop dividing, becoming senescent. When cells become senescent changes in morphology and gene expression patterns occur. In addition, senescence is also accompanied by increases in acidic senescence-associated β-galactosidase (SA-ÃŸ-Gal). This activity can be monitored using the substrate 5-bromo-4-chloro-3-indolyl-β-galactopyranoside (X-Gal) at pH 6 and is a useful enzymatic marker of cellular senescence (SA-β-Gal). While cells entering senescence show this enzymatic activity, immortalized cell types, including tumor or other quiescent cells, are not stained under these conditions. The implications of these methods in such diverse areas as age-related pathology research, tumor analysis and tissue culture maintenance is significant. When β-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl derivative is produced, which quickly dimerizes and becomes oxidized to produce a bright blue indigo dye precipitate at the site of β-Gal activity. The dimerization and oxidation reaction is facilitated, in this kit, by a developer buffer solution containing ferric and ferrous ions. Senescent cells can be determined by examining the number of blue cells in the total cell population. The kit provides enough reagents and standards for up to 100 assays, using a microtiterplate method, and includes a detailed protocol for use in cultured mammalian cells.
Contact us about this product :
Our team will respond you as soon as possible !
We have also other products like : MarkerGene™ Cellular Senescence Assay Kit, Can be used to monitor and detect â_galactosidase activity in senescent cells at pH 6.0 using X_Gal, 1 kit
52062 Aachen Deutschland
Support Karolina Elandt
Fax: (+49) 241 56 00 47 88
Logistic :0241 40 08 90 86
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
email@example.com | Gentaur
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Finland Helsset +358942419041
Magyarország Budapest +3619980547
US New York+17185132983
WP1004: Kit Receptor Signaling Pathway
WP1121: Kit Receptor Signaling Pathway
WP1341: Kit Receptor Signaling Pathway
WP147: Kit Receptor Signaling Pathway
WP1603: Nicotine Activity on Chromaffin Cells
WP407: Kit Receptor Signaling Pathway
WP548: neural crest development
WP774: Kit Receptor Signaling Pathway
WP886: Kit Receptor Signaling Pathway
WP1058: Senescence and Autophagy
WP1174: Senescence and Autophagy
WP1267: Senescence and Autophagy
WP1305: Senescence and Autophagy
WP1378: Senescence and Autophagy
WP1455: Serotonin Transporter Activity
WP1488: CFTR activity in the plasma membrane
WP1601: Fluoropyrimidine Activity
WP1602: Nicotine Activity on Dopaminergic Neurons
WP1765: Mechanosensory inputs influence pharyngeal activity via ivermectin sensitivity genes.
WP1872: Neurotransmitter uptake and Metabolism In Glial Cells
WP1899: Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs)
WP2083: Nicotine Activity on Dopaminergic Neurons
WP2231: Degradation of apoptotic cells
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP259: Nifedipine Activity
Related Genes :
 Rapid diagnostic test for G6PD deficiency in Plasmodium vivax infected men: a budget impact analysis based in Brazilian Amazon.
 An efficacy and safety of nanofractional radiofrequency for the treatment of striae alba.
 Familiarity expands space and contracts time.
 Analysis of DNA Hydroxymethylation Using Colorimetric Assay.
 Control of Cortaderia selloana with a glyphosate-based herbicide led to a short-term stimulation of soil fungal communities.
 NRG oncology RTOG 0625: a randomized phase II trial of bevacizumab with either irinotecan or dose-dense temozolomide in recurrent glioblastoma.
 Deficiency in Outer Dense Fiber 1 is a marker and potential driver of idiopathic male infertility.
 Duration of antibody response following vaccination against feline immunodeficiency virus.
 Vaccine development: From concept to early clinical testing.
 Association between Postdischarge Oral Corticosteroid Prescription Fills and Readmission in Children with Asthma.
Enter catalog number :