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Index / MarkerGene / Nile Red, A lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells, 10 mg / Product Detail : M1441 Nile Red, A lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells, 10 mg
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#M1441 Nile Red, A lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells, 10 mg

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  Price : 148   EUR
168   USD
115   GBP
624   Zloty
19896   JPY
1146   NOK
1228   SEK
168   CHF

Product name : Nile Red, A lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells, 10 mg

Catalog number : M1441

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Availability: Yes

Supplier name : MarkerGene

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More Details about

Description:
Lipophilic dye that stains intracellular lipid droplets to produce a bright red fluorescence in live cells.

Application:
Nile red is almost nonfluorescent in water but becomes intensely fluorescent, with a strong red emission when in a lipid-rich environment (EX552 nm/EM636 nm). But the fluorescence emission is environmentally sensitive. Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (EX 485 nm/EM 525 nm) while polar lipids, such as phospholipids, will fluoresce red. The ratio of green:red fluorescence can be used to normalize cell size and dye uptake and to identify cells that have more "fat vacuoles". The probe can be added directly to the cell culture in order to label the fat vacuoles within adipocytes and these can then be analyzed by flow cytometry. Nile red has also been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. For tissue staining, a stock solution of Nile red (500 ug/mL) in acetone is prepared and stored chilled and protected from light. A fresh staining solution of Nile red is made by the addition of 2-10 uL of the stock solution to 1 ml of 75% glycerol followed by brisk vortexing. The glycerol-dye solution is then briefly degassed by vacuum to remove bubbles. To stain frozen sections of tissue, a drop of the glycerol staining solution is added to each section and the preparation covered with a glass coverslip. After 5 min., the sections can be examined by fluorescence microscopy. Depending on the amount of tissue lipid present and the tissue thickness, the amount of Nile red required may need to be adjusted for optimum staining. Typically 1-5 ug dye/ml of 75% glycerol is required for maximum fluorescent staining.

Molecular Weight: 318.37

Storage: RT, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: CHCl3, DMSO

Absorption: (in nm) 530

Emission: (in nm) 635

Extinction (103): 38

Molecular Formula: C20H18N2O2

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