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Index / Sceti K.K. / E.coli LexA repressor / Product Detail : 01-006 E.coli LexA repressor
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Aug
25th

#01-006 E.coli LexA repressor

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  Price : 1445   EUR
1640   USD
1121   GBP
6071   Zloty
193382   JPY
11145   NOK
11942   SEK
1634   CHF

Product name : E.coli LexA repressor

Catalog number : 01-006

Quantity: 100ug

Availability: Yes

Supplier name : Sceti K.K.

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More Details about

Description
E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are
related to DNA repair and cell division by recognizing and binding to the SOS-box sequence
(TACTGTATATATATACAGTA). LexA‘s self-protease activity is promoted by RecA protein which,
responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells.
It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of
genes belonging to the SOS regulon is induced, and DNA repair ability and mutagenic activity in the
cells are enhanced (1).
LexA fused genes are used as baits in the experiments to detect the protein-protein interaction in the
yeast two-hybrid method (2).
The product is over-produced as a recombinant protein, and highly purified by several steps of
chromatography. A single band is observed by SDS-PAGE at 23 kD (Fig 1).


Usage
• Studies on the mechanism of E. coli SOS response.
• Used as an antigen for positive control in Western blotting to confirm that the Bait construct is
expressed stably in the nucleus as protein of the expected size in the yeast two-hybrid method
using the LexA gene. See also antibody to LexA protein (#61-001, #61-002)


Specification
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.8 mg/ml* as measured by BCA method
Form: 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Storage: -20c


Reference
1. Waker GC, Cold Spring Harb. Symp. Quant. Biol. 65:1-10 (2000)
2. Sambrook J & Russell DW, Molecular Cloning 3 rd Ed. Chapter 18.17-18.27 (2001) Cold Spring Harber Laboratory Press

Fig. 1: Polyacrylamide gel electrophoresis of LexA protein.

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