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Index / Sceti K.K. / HIV_1 Gag p24 / Product Detail : 05-005 HIV_1 Gag p24
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Jul
26th

#05-005 HIV_1 Gag p24

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  Price : 434   EUR
493   USD
336   GBP
1824   Zloty
58114   JPY
3349   NOK
3589   SEK
491   CHF

Product name : HIV_1 Gag p24

Catalog number : 05-005

Quantity: 20ug

Availability: Yes

Supplier name : Sceti K.K.

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More Details about

Description
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus, HIV-1, and is produced by
digestion of its precursor, Gag p55, by HIV-1 protease. This protein is indispensable for reproduction
of AIDS virus and constitutes an essential element in the virus particle (1). As this protein is
detectable from the early stage of AIDS virus infection, and reflects the amount of virus in the blood,
it is used as a marker for observing the patient’s condition during and after treatment.
This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag
p24 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of
chromatography (3). Its molecular weight is 24 kD, same as that of p24 purified from HIV-1 virus
particles (Fig 1).


Usage
1) It can be used as a standard in titration of p24 antigens as it indicates the amount of HIV-1 virus.
This measurement is useful for diagnosis of virus infection and assessing the amount of virus
during and after treatment.
2) It can be used as antigen for Western blotting or ELISA of anti-HIV-1 p24 antibody.
3) It can be used in the studies of structure and function of HIV-1 virus as it constitutes HIV-1 core as
a capsid protein.


Specification
Purity: Over 90% purity by SDS-PAGE (CBB staining)
Protein concentration: 1 mg/ml* measured by BCA method
Form: 50% glycerol, 20mM Tris-HCl (pH7.5), 50mM NaCl, 10mM mercaptoethanol
Storage: -20°C

Fig.1 Polyacrylamide gel electrophoresis of HIV-1 p24 protein

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