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Index / Sceti K.K. / HIV_1 Nef / Product Detail : 05-012 HIV_1 Nef
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Dec
5th

#05-012 HIV_1 Nef

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  Price : 1113   EUR
1264   USD
864   GBP
4678   Zloty
149008   JPY
8588   NOK
9202   SEK
1259   CHF

Product name : HIV_1 Nef

Catalog number : 05-012

Quantity: 100ug

Availability: Yes

Supplier name : Sceti K.K.

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More Details about

Description
HIV-1 Nef is one of the accessory proteins synthesized in the early stage of HIV-1 reproduction and
is abundantly found in infected cells. The name derives from its negative factor thought at the
beginning but presently it is remarked as the protein which bears a most distinctive biological
characteristic of HIV-1 (1). The protein interacts directly with the signal transduction protein of the
host T cell and works effectively on AIDS infection or on long term survival of the infected cells or
induces apoptosis of non-infected cells (2). It is also involved in the endocytosis and degradation of
receptor protein of the cell surface such as CD4 and MH4, important for HIV infection.
The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the nef
gene of HIV-1 virus, subtype B(3) and highly purified by several steps of chromatography. Its
molecular size is 27kD, same as that of Nef purified from HIV-1 viral particles (Fig 1).


Usage
• For functional studies of HIV-1 Nef protein.
• Used as standard for the titration analysis of HIV-1 Nef antigen.
• It can be used as Nef antigen in detection of anti-HIV-1 Nef antibody by Western blotting or ELISA.


Specification
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.48 mg/ml* as determined by BCA method
Form: 50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Storage: -20 oC

Fig. 1 : Polyacrylamide gel electrophoresis of HIV-1 Nef protein.

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