GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908,

Index / Geba / Antibody Stripping Buffer / Product Detail : ST010 Antibody Stripping Buffer
Related keywords:


#ST010 Antibody Stripping Buffer

Ask technical file .

  Price : 75   EUR
86   USD
58   GBP
319   Zloty
10162   JPY
585   NOK
627   SEK
85   CHF

Product name : Antibody Stripping Buffer

Catalog number : ST010

Quantity: 1

Availability: Yes

Supplier name : Geba

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Cat. No.  Description
ST010  Immunodetection removing buffer, 500 ml 
ST013  Immunodetection removing buffer, 30 ml 
Storage and Shipping Instruction:  Shipped at ambient temperature. Upon arrival,
store at 4oC. Stable at room temperature or 4oC for 1 year.
IMPORTANT: Allow the buffer to warm to room temperature before use.
This product is guaranteed for one year from date of purchase when properly handled and stored.

Western Blot is widely used to detect and compare proteins from a complex mixture utilizing antibody detection on a membrane. Chemiluminescence has become an easy and sensitive method of detection compared to other methods of analysis. Because of the nature of chemiluminescence detection, it is possible to  reprobe the separated protein mixture on the membrane. Conventionally, Western blots have been stripped using extremely harsh conditions that  may alter the antigen for subsequent immunoprobing. The Gene Bio-Application  Stripping Buffer is a novel formula
providing a gentle method of removing  primary and secondary antibodies from membranes that allows reprobing the same membrane several times.   

IMPORTANT: Optimization of incubation time is essential for best results.
IMPORTANT: If the blot cannot  be stripped immediately after chemiluminescence detection, the  blot can be stored in PBS or
TBST at 4o C until the stripping procedure is performed. 
1.  Place the blot to be stripped in the Stripping Buffer. Top up Stripping
Buffer until the blot is completely immersed.

2. Incubate the blot in the Striping buffer for 5-15 min at room temperature
under vigorous shaking.
IMPORTANT: In general, higher  affinity antibodies or large quantities of detected protein will require longer incubation time for stripping. However, each half-minute is critical to achieve optimum incubation time.
3. Empty the Stripping Buffer. 
4. To wash, add 300 ml of dH2O and shake vigorously for 5 min.
Repeat Step 4 five more times.
Verification of complete removal of the HRP label: After Step
5 incubate the membrane with  fresh chemiluminescence reagent and expose to film. If no signal is detected after 5 min exposure, the HRP conjugate has been  successfully removed from the
antigen or primary antibody.
Verification of complete removal of primary label antibody:
After Step 5 incubate the membrane with the HRP-labeled secondary antibody, followed by a wash in wash buffer. Incubate the membrane with fresh chemiluminescence reagent and expose
to film. If no signal is detected after 5 min exposure, the primary antibody has been successfully removed from the antigen.
IMPORTANT:  Analysis of successful removal of immunoprobes is recommended to prevent removal of the antigen or the unsuccessful removal of the antibodies. 
6. If signal is detected in the two experiments described above, place the blot again in Stripping Buffer for additional 5-15 min.
7. After it has been determined that the membrane is free of the immunodetection reagents, a second immunoprobe can take place.
Start the second immunoprobe with reblocking of the blot. 
IMPORTANT: The blot can be stripped up to 5 times.
However, longer exposure times or more sensitive chemiluminescence substrate is  needed. Actually, re-probing may result in a decrease in signal if the antigen is unstable.
Analysis of the individual system is required.  

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Geba \ Antibody_Stripping_Buffer \ ST010
Reload Image


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45 | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411 | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925 | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897 | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

tel:0911876558 | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48       | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94 | Gentaur