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#51-003 removed without changing the original DNA sequence. As such, it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a

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Product name : removed without changing the original DNA sequence. As such, it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a

Catalog number : 51-003

Quantity: 100 ug

Availability: Yes

Supplier name : BBridge

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DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. As such, it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — in this case with the specific effect of reducing gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.

Applications
1) Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
2) Immuno-blotting detection of DNA with 5-methylocytosine on nitrocellulose (Ref. 3 & 4) (~1000
fold dilution).

Specifications
Immunogen: 5-Methylcytosine conjugated to bovine serum albumin (Ref 3)
Reactivity: DNA with 5-Methylcytosine (methylated DNA), any species
Isotype: IgM
Form: Purified mouse IgM 1 mg/ml in PBS with 50% glycerol, filter-sterilized
Storage: -20oC (long term storage, -80 oC)

Fig.1. Methylation of chloroplast DNA visualized by immunochemistry.
Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells.
Middle: Cells stained with anti-5MeC antibody and FITC-conjugated
2nd antibody. Right: Merged image. Chloroplast DNA is exclusively
methylated in gamete cells.

Fig. 2. Detection of DNA methylation in mouse embryonic stem cells by
immunofluorescece staining with the anti-5MeC antibody. Intense
5-methylcytosine staining at pericentromeric regions was seen in the
mitotic chromosome and interphase nuclei of ESCs. (see Reference
1.).

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