GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Biotix / 200 µl Extended Length Gel Tip, Non_Filtered, Non_Sterile, Standard, Racked 204 / Product Detail : 2016 200 µl Extended Length Gel Tip, Non_Filtered, Non_Sterile, Standard, Racked 204
Related keywords:

Jul
29th

#2016 200 µl Extended Length Gel Tip, Non_Filtered, Non_Sterile, Standard, Racked 204

Ask technical file .

  Price : 552   EUR
626   USD
428   GBP
2318   Zloty
73860   JPY
4257   NOK
4561   SEK
624   CHF

Product name : 200 µl Extended Length Gel Tip, Non_Filtered, Non_Sterile, Standard, Racked 204

Catalog number : 2016

Quantity: 5100

Availability: Yes

Supplier name : Biotix

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2HPO4)
APPLICATION: Ideal as an ELISA antigen for detecting antibodies specific to mouse type II collagen in sera from experimental animals and humans, such as collagen-induced arthritis (CIA) models and patients with arthritis or other diseases, as well as normal controls.
QUANTITY: 1 mL
FORM: 0.5 mg/mL solution in 0.05M acetic acid
SOURCE: Mouse sternal cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
USAGE: It is recommended to dilute this product with chilled Collagen Dilution Buffer, (catalog # 9003) provided with the ELISA grade collagen, to prevent the aggregation of collagen molecules. For coating, takealiquots and dilute it with the Collagen Dilution Buffer just before use. Do not keep diluted solutions.
The optimum concentration of collagen to coat ELISA plates is 5 mg/mL generally, but may vary based
on the type of plate used for different purposes such as coating cell culture plates.
STORAGE TEMPERATURE: -20°C in the dark. This product is stable in acidic conditions at 4°C for several weeks and at -20°C for oneyear as supplied, but will gradually form polymeric collagen and degrade under neutral conditions.
STABILITY: 1 year

 

Chondrex provides highly purified, polymeric-free ELISA grade collagen. Various species and types of collagen can be used as an antigen in ELISA for studying antibody specificity and cross-reactivity (Table 1). Since collagen is a rigid fibrilar protein with unique physical and chemical properties and differs from other globular proteins, special attention for handling this protein is required. For example, immunoglobulins in human and animal sera bind to polymeric and fibrilar collagen non-specifically, and create significantly high false-positive reaction in ELISA.

Similarly, a secondary antibody in ELISA also binds to fibrilar collagen non-specifically and creates high background values. Furthermore, these forms of collagen affect the assay sensitivity significantly due to the formation of excess collagen layer on the surfaces of ELISA plate.


ELISA grade collagen is supplied as a 100X concentrated solution (0.5 mg/mL). Each vial contains enough collagen to coat ten, 96-well plates.
A 10X Collagen Dilution Buffer (catalog # 9003) is also included per order of ELISA grade collagen to prevent fibril formation.

 

PROCEDURE FOR COATING elISA PLATES
1. Dilute 1 mL of 10X Collagen Dilution Buffer with 9 mL of cold (refrigerated) glass distilled water.
2. Add 100 mL of ELISA grade type I or type II collagen solution to 10 mL of 1X collagen dilution buffer prepared in step 1.
3. Add 100 mL of the diluted collagen solution to each well and incubate at 4°C overnight. Cover the ELISA plate to prevent evaporation.
Note: The binding efficiency of collagen on ELISA plates depends upon the type of plastic and pre-treatment of the surface. Therefore, we
recommend using a high binding plate such as Immulon 2 HB. Mouse monoclonal anti-type II collagen antibody (catalog # 7005), which cross-reacts to all species of type II collagen listed in Table 1, can be used as a positive control or to study the binding efficiency of individual species of type II collagen on ELISA plates.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Biotix \ 200_µl_Extended_Length_Gel_Tip,_Non_Filtered,_Non_Sterile,_Standard,_Racked_204 \ 2016
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur