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Index / EpigenTek / EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K4 / Product Detail : P-3002-2 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K4
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#P-3002-2 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K4

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  Price : 704   EUR
799   USD
546   GBP
2957   Zloty
94186   JPY
5428   NOK
5816   SEK
796   CHF

Product name : EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K4

Catalog number : P-3002-2

Quantity: 96 assays

Availability: Yes

Supplier name : EpigenTek

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More Details about

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4)

Product Overview

The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K4) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 4 (H3-K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment.

WHY CHOOSE THE EPIQUIK™ HISTONE METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT (H3-K4)?

  • Very rapid procedure, which can be completed within 3 hours.
  • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • Specific measurement of activity/inhibition of H3-K4 histone methyltransferases.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Extremely simple, reliable, and consistent assay conditions.

 

Product Components

HM1 (10X Wash Buffer)
HM2 (Histone Assay Buffer)
HM3 (Adomet)*
HM4 (Biotinylated Substrate, 25 µg/ml)*
HM5 (HMT Standard, 10 µg/ml)*
HM6 (Capture Antibody, 100 µg/ml)*
HM7 (Detection Antibody, 200 µg/ml)*
HM8 (Developing Solution)
HM9 (Stop Solution)
Control Enzyme (100 µg/ml)*
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

Frequently Asked Q's


1. What is the principle of the assay in kit P-3002?
The histone H3 substrate is first captured to the assay wells. H3-K4 site-specific enzymes transfer the methyl group from Adomet to histone H3 at lysine 4 and methylate this lysine site. The methylated H3-K4 can then be recognized with an anti-dimethyl H3-K4 antibody. The ratio or amount of methylated H3-K4, which is proportional to enzyme activity, can be colorimetrically quantified through an ELISA-like reaction.

2. Is kit P-3002 suitable for rat samples?
Yes.

3. Is the assay done in 8-well strips transferable to an older plate reader?
The solution in the 8-well strips can be transferred to the 96-well plate for reading. Also the 8-well strips can be put in the 96-well plate size frame (included in the kit) and directly read with the plate reader.

4. Is it HMT enzyme that is used to obtain the standard curve in this kit?
In the P-3002 kit, histone methyltransferase (HMT)-converted products are used for standard curve and HMT is only used as the positive control.

 

Product Citations

Rugg-Gunn, PJ. et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci USA. 107(24):10783-90. PubMed Abstract Full PDF Article

Sebastian, S. et. al. (March 2009). MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation. Proceedings of the National Academy of Sciences. 160(12): 4719-24.

 

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