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Index / EpigenTek / Methylamp Global DNA Methylation Quantification Ultra Kit / Product Detail : P-1014B-96 Methylamp Global DNA Methylation Quantification Ultra Kit
#P-1014B-96 Methylamp Global DNA Methylation Quantification Ultra Kit
Product name : Methylamp Global DNA Methylation Quantification Ultra Kit
Catalog number : P-1014B-96
Quantity: 96 assays
Supplier name : EpigenTek
Data sheet: Ask more or other datasheet now !
About this Product :Methylamp Global DNA Methylation Quantification Ultra Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Methylamp Global DNA Methylation Quantification Ultra Kit .
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We recommend using a newer version of this product: #P-1034 MethylFlash Methylated DNA Quantification Kit (Colorimetric)
The Methylamp™ Global DNA Methylation Quantification Ultra Kit is an improved version of the popular Methylamp™ Global DNA Methylation Quantification Kit. As a complete set of essential components, the kit enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed in less than 4 hours in either manual or high throughput analysis. In addition to having a shorter procedure, the improved kit also has higher sensitivty than its predecessor. It is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc. The universal positive control included in the kit allows for quantitation of methylated DNA from any species.
WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?
- Colorimetric quantification without radioactivity, extraction, and chromatography.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
IMPROVEMENTS OVER ITS PREDECESSOR:
- Shorter procedure, which can be finished in less than 4 hours.
- Significantly higher sensitivity -- detection limitation can be as low as 0.2 ng of methylated DNA.
- Universal positive control suitable for quantifying methylated DNA from any species.
The Methylamp™ Global DNA Methylation Quantification Ultra Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with DNA affinity substance. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.
Quantification of Methylated DNA.
GU1 (10X Wash Buffer)
GU2 (DNA Binding Solution)
GU3 (Positive Control,100 µg/ml)**
GU4 (Block Solution)
GU5 (Capture Antibody, 1000 µg/ml)*
GU6 (Detecting Antibody, 400 µg/ml)*
GU7 (Enhance Solution)
GU8 (Developing Solution)
GU9 (Stop Solution)
NC (Negative Control)
8-Well Assay Strips (with Frame)
* Spin the solution down to the bottom before use.
** This control is synthesized polynucleiotide methylated at every 5-cytosine.
McGuinness, D. et. al. (January 2012). Socio-economic status is associated with epigenetic differences in the pSoBid cohort. Int J Epidemiol. Epub ahead of print. PubMed Abstract
Shearstone, JR. et. al. (November 2011). Global DNA demethylation during mouse erythropoiesis in vivo. Science. 334(6057):799-802 PubMed Abstract
Birkus, G. et. al. (May 2011). Role of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191. Antimicrob Agents Chemother. 55(5)):2166-73 PubMed Abstract
Shi, Y. Y. et. al. (April 2011). Diet and Cell Size Both Affect Queen-Worker Differentiation through DNA Methylation in Honey Bees (Apis mellifera, Apidae). PLoS One. 6(4): e18808. PubMed Abstract
Desplats, P. et. al. (February 2011). Alpha-synuclein sequesters DNMT1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. Epub ahead of print. PubMed Abstract
Huang, S. K. et. al. (November 2010). Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. Am J Pathol. 177(5): 2245-55. PubMed Abstract
Liu, C. et. al. (November 2010). Global DNA Methylation, DNMT1, and MBD2 in Patients with Systemic Lupus Erythematosus. Lupus. 20(2): 131-6. PubMed Abstract
Vucetic, Z. et. al. (October 2010). Maternal high-fat diet alters methylation and gene expression of dopamine and opioid-related genes. Endocrinology. 151(10): 4756-64. PubMed Abstract
LaPlant, Q. et. al. (September 2010). Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nat Neurosci. 13(9): 1137-43. PubMed Abstract
Sanders, Y. Y. et. al. (August 2010). Epigenetic Regulation of Thy-1 by Histone Deacetylase Inhibitor in Rat Lung Fibroblasts. Am J Respir Cell Mol Biol. Epub ahead of print. PubMed Abstract
Li, Y. et. al. (May 2010). Overexpression of the growth arrest and DNA damage-induced 45 gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells. Arthritis & Rheumatism. 62(5): 1438-1447. PubMed Abstract
Wang, L. et. al. (February 2010). Relation between hypomethylation of long interspersed nucleotide elements and risk of neural tube defects. Am. J. Clin. Nutr. 91(5): 1359-67. Full PDF Article
Yamanaka, K. l. et. al. (February 2010). Gene Silencing of DNA Methyltransferases by RNA Interference in Bovine Fibroblast Cells. J Reprod Dev. 56(1): 60-7. Full PDF Article
Shutoh, Y. et. al. (October 2009). Low dose effects of dichlorodiphenyltrichloroethane (DDT) on gene transcription and DNA methylation in the hypothalamus of young male rats: implication of hormesis-like effects. Journal of Toxicological Sciences. 34(5): 469-482. PubMed Abstract
Blazkova, J. et. al. (August 2009). CpG methylation controls reactivation of HIV from latency. PLoS Pathogens. 5(8): e1000554. Full PDF Article
Chen, C. et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Investigation. 27(5): 582-91. PubMed Abstract
Mahmoud, W. F. et. al. (March 2009). AtMBD9 modulates Arabidopsis development through the
dual epigenetic pathways of DNA methylation and histone acetylation. The Plant Journal. 59: 123-135. Full PDF Article
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WP2077: Methylation Pathways
WP704: Methylation Pathways
Related Genes :
 Aberrant DNA methylation patterns of spermatozoa in men with unexplained infertility.
 Overexpression of DNA damage-induced 45 α gene contributes to esophageal squamous cell cancer by promoter hypomethylation.
 Socio-economic status is associated with epigenetic differences in the pSoBid cohort.
 Clinical and public health research using methylated DNA immunoprecipitation (MeDIP): a comparison of commercially available kits to examine differential DNA methylation across the genome.
 Overexpression of the growth arrest and DNA damage-induced 45alpha gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells.
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