GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / EpigenTek / Methylamp Global DNA Methylation Quantification Ultra Kit / Product Detail : P-1014B-96 Methylamp Global DNA Methylation Quantification Ultra Kit
Related keywords:

Aug
28th

#P-1014B-96 Methylamp Global DNA Methylation Quantification Ultra Kit

Ask technical file .

  Price : 739   EUR
839   USD
573   GBP
3105   Zloty
98909   JPY
5700   NOK
6108   SEK
836   CHF

Product name : Methylamp Global DNA Methylation Quantification Ultra Kit

Catalog number : P-1014B-96

Quantity: 96 assays

Availability: Yes

Supplier name : EpigenTek

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

Methylamp Global DNA Methylation Quantification Ultra Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Methylamp Global DNA Methylation Quantification Ultra Kit .
http://mybiofast.com/ver.php?search=anti-&submit=Search

More Details about

We recommend using a newer version of this product: #P-1034 MethylFlash Methylated DNA Quantification Kit (Colorimetric)

The Methylamp™ Global DNA Methylation Quantification Ultra Kit is an improved version of the popular Methylamp™ Global DNA Methylation Quantification Kit. As a complete set of essential components, the kit enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed in less than 4 hours in either manual or high throughput analysis. In addition to having a shorter procedure, the improved kit also has higher sensitivty than its predecessor. It is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc. The universal positive control included in the kit allows for quantitation of methylated DNA from any species.

WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?

  • Colorimetric quantification without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.


IMPROVEMENTS OVER ITS PREDECESSOR:

  • Shorter procedure, which can be finished in less than 4 hours.
  • Significantly higher sensitivity -- detection limitation can be as low as 0.2 ng of methylated DNA.
  • Universal positive control suitable for quantifying methylated DNA from any species.
Product Details

The Methylamp™ Global DNA Methylation Quantification Ultra Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with DNA affinity substance. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.
 

SCHEMATIC PROCEDURE:  



Quantification of Methylated DNA.
Product Components

GU1 (10X Wash Buffer)
GU2 (DNA Binding Solution)
GU3 (Positive Control,100 µg/ml)**
GU4 (Block Solution)
GU5 (Capture Antibody, 1000 µg/ml)*
GU6 (Detecting Antibody, 400 µg/ml)*
GU7 (Enhance Solution)
GU8 (Developing Solution)
GU9 (Stop Solution)
NC (Negative Control)
8-Well Assay Strips (with Frame)
User Guide

* Spin the solution down to the bottom before use.
** This control is synthesized polynucleiotide methylated at every 5-cytosine.

Product Citations

McGuinness, D. et. al. (January 2012). Socio-economic status is associated with epigenetic differences in the pSoBid cohort. Int J Epidemiol. Epub ahead of print. PubMed Abstract

Shearstone, JR. et. al. (November 2011). Global DNA demethylation during mouse erythropoiesis in vivo. Science. 334(6057):799-802 PubMed Abstract

Birkus, G. et. al. (May 2011). Role of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191. Antimicrob Agents Chemother. 55(5)):2166-73 PubMed Abstract

Shi, Y. Y. et. al. (April 2011). Diet and Cell Size Both Affect Queen-Worker Differentiation through DNA Methylation in Honey Bees (Apis mellifera, Apidae). PLoS One. 6(4): e18808. PubMed Abstract

Desplats, P. et. al. (February 2011). Alpha-synuclein sequesters DNMT1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. Epub ahead of print. PubMed Abstract

Huang, S. K. et. al. (November 2010). Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. Am J Pathol. 177(5): 2245-55. PubMed Abstract

Liu, C. et. al. (November 2010). Global DNA Methylation, DNMT1, and MBD2 in Patients with Systemic Lupus Erythematosus. Lupus. 20(2): 131-6. PubMed Abstract

Vucetic, Z. et. al. (October 2010). Maternal high-fat diet alters methylation and gene expression of dopamine and opioid-related genes. Endocrinology. 151(10): 4756-64. PubMed Abstract

LaPlant, Q. et. al. (September 2010). Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nat Neurosci. 13(9): 1137-43. PubMed Abstract

Sanders, Y. Y. et. al. (August 2010). Epigenetic Regulation of Thy-1 by Histone Deacetylase Inhibitor in Rat Lung Fibroblasts. Am J Respir Cell Mol Biol. Epub ahead of print. PubMed Abstract

Li, Y. et. al. (May 2010). Overexpression of the growth arrest and DNA damage-induced 45 gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells. Arthritis & Rheumatism. 62(5): 1438-1447. PubMed Abstract

Wang, L. et. al. (February 2010). Relation between hypomethylation of long interspersed nucleotide elements and risk of neural tube defects. Am. J. Clin. Nutr. 91(5): 1359-67. Full PDF Article

Yamanaka, K. l. et. al. (February 2010). Gene Silencing of DNA Methyltransferases by RNA Interference in Bovine Fibroblast Cells. J Reprod Dev. 56(1): 60-7. Full PDF Article 

Shutoh, Y. et. al. (October 2009). Low dose effects of dichlorodiphenyltrichloroethane (DDT) on gene transcription and DNA methylation in the hypothalamus of young male rats: implication of hormesis-like effects. Journal of Toxicological Sciences. 34(5): 469-482. PubMed Abstract


Blazkova, J. et. al. (August 2009). CpG methylation controls reactivation of HIV from latency. PLoS Pathogens. 5(8): e1000554. Full PDF Article

Chen, C. et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Investigation. 27(5): 582-91. PubMed Abstract

Mahmoud, W. F. et. al. (March 2009). AtMBD9 modulates Arabidopsis development through the
dual epigenetic pathways of DNA methylation and histone acetylation. The Plant Journal. 59: 123-135. Full PDF Article

 

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
EpigenTek \ Methylamp_Global_DNA_Methylation_Quantification_Ultra_Kit__ \ P_1014B_96
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur