GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / EpigenTek / EpiQuik Whole Cell Extraction Kit / Product Detail : OP-0003-100 EpiQuik Whole Cell Extraction Kit
Related keywords:

Aug
30th

#OP-0003-100 EpiQuik Whole Cell Extraction Kit

Ask technical file .

  Price : 194   EUR
221   USD
151   GBP
817   Zloty
26051   JPY
1501   NOK
1608   SEK
220   CHF

Product name : EpiQuik Whole Cell Extraction Kit

Catalog number : OP-0003-100

Quantity: 100 assays

Availability: Yes

Supplier name : EpigenTek

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

The EpiQuik™ Whole Cell Extraction Kit provides the simple and selective method for extracting whole cell proteins used for a variety of applications. These applications may include western blotting, cellular enzyme assays, and others requiring cellular proteins. The EpiQuik™ Whole Cell Extraction Kits are also specifically designed to meet the requirements of whole cell extracts used in EpiQuik™ assays. The EpiQuik™ Whole Cell Extraction Kit can be used to extract cellular proteins from mammalian cells and tissue samples. The EpiQuik™ Whole Cell Extraction Kit has the fastest procedure available on the current market that can be finished within 45 minutes.

 

Product Details

The EpiQuik™ Whole Cell Extraction Kit provides the simple and selective method for extracting whole cell proteins used for a variety of applications. These applications may include western blotting, cellular enzyme assays, and others requiring cellular proteins. The EpiQuik™ Whole Cell Extraction Kits are also specifically designed to meet the requirements of whole cell extracts used in EpiQuik™ assays. The EpiQuik™ Whole Cell Extraction Kit can be used to extract cellular proteins from mammalian cells and tissue samples. The EpiQuik™ Whole Cell Extraction Kit has the fastest procedure available on the current market that can be finished within 45 minutes.
 

 
 




Whole cell extracts were prepared from MCF-7 cells using this kit. Activity of SOD was measured using different amounts of the extract.

Product Components

5X Extraction Buffer
1000X DTT Solution
1000X Protease Inhibitor Cocktail (PIC)
User Guide

 

INTRODUCTION
The EpiQuik™ Whole Cell Extraction Kit provides a simple and selective method
for extracting whole cell proteins used for a variety of applications. These
applications may include western blotting, cellular enzyme assays, and others
requiring cellular proteins. The EpiQuik™ Whole Cell Extraction Kits are also
specifically designed to meet the requirements of whole cell extracts used in
EpiQuik™ assays. The EpiQuik™ Whole Cell Extraction Kit can be used to extract
cellular proteins from mammalian cells. The EpiQuik™ Whole Cell Extraction Kit
has the fastest procedure available on the current market that can be finished
within 45 minutes.
PRODUCT USE INFORMATION
The EpiQuik™ Whole Cell Extract Kit is very suitable for quick preparation of
whole cell extracts from mammalian cells and tissue samples.
The EpiQuik™ Whole Cell Extract Kit is recommended for use with EpiQuik™
Superoxide Dismutase Assay/Inhibition assay kit.
The EpiQuik™ Whole Cell Extract Kit is for research use only and is not intended
for diagnostic or therapeutic application.
The EpiQuik™ is a trademark of Epigentek, Inc.

SHIPPING AND STORAGE
Store all components at 4ºC. The kit is stable for up to 1 year from date of shipment
under this storage condition.

PROTOCOL
Before starting, ensure that 5X extraction buffer is in clear solution. Warm to room
temperature and shake or vortex if the buffer precipitates.
Cell Pellet Preparation
For Monolayer or Adherent Cells:
1. Cells (treated or untreated) are grown to 80-90% confluence and
trypsinized after removing growth medium. Cells are then collected into
a 15 ml conical tube and counted in a hemacytometer.
2. Cells are washed once with PBS and pelleted by centrifugation at 1000
rpm for 5 min.
For Non-adherent Cells:
1. Grow cells to 2 X 106 /ml and collect the cells into a 15 ml conical tube.
2. Centrifuge the cells for 5 min at 1000 rpm and discard the supernatant.
Wash cells with PBS once by centrifugation at 1000 rpm for 5 min. Discard
the supernatant.

 

Cell Extract Preparation
1. Prepare 1X extraction buffer by adding 1 ml of 5X extraction buffer to 4
ml of distilled water.
2. Add DTT solution and PIC to 1X extraction buffer at the 1: 1000 ratios.
Re-suspend cell pellet in 100 μl of ice cold 1X extraction buffer per 106
adherent cells or 2 x 106 non-adherent cells.
3. Transfer the cell solution to a micro centrifuge vial. Incubate on ice for
15 min with vigorous vortex (5 sec) per 5 min.
4. Centrifuge the cell solution for 10 min at 14,000 rpm at 4ºC and transfer
the supernatant into a new microcentrifuge vial.
5. Measure the protein concentration of cell extract.
6. Use immediately or aliquot and freeze supernatant at –70ºC until further
use. Avoid freeze/thaw cycle.

 

Available Related Products
EpiQuik™ Nuclear Extraction Kit I 100 extractions OP-0002-1
EpiQuik™ Nuclear Extraction Kit II 100 extractions OP-0002-1

 

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
EpigenTek \ EpiQuik_Whole_Cell_Extraction_Kit__ \ OP_0003_100
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur