GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, firstname.lastname@example.org
Index / EpigenTek / EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27 / Product Detail : P-3005-48 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
#P-3005-48 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
Product name : EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
Catalog number : P-3005-48
Quantity: 48 assays
Supplier name : EpigenTek
Data sheet: Ask more or other datasheet now !
More Details about
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3-K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment.
WHY CHOOSE THE EPIQUIK™ HISTONE METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT (H3-K27)?
- Very rapid procedure, which can be completed within 3 hours.
- Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
- Specific measurement of activity/inhibition of H3-K27 histone methyltransferases.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Extremely simple, reliable, and consistent assay conditions.
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is designed for measuring HMTs that specifically target histone H3 at lysine 27. In an assay with this kit, the histone substrate is stably captured on the strip wells through biotin-streptavidin binding. HMT enzymes transfer a methyl group to histone H3 substrate from Adomet to methylate the substrate at lysine 4. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K27, which is directly proportional to enzyme activity, can be quantified through a HRP conjugated secondary antibody-color development system. The HMT activity is then calculated based on the amount of methylated H3-K27 converted by the HMTs.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and H3-K27 specific histone methyltrasferase activity was measured.
HE1 (10X Wash Buffer)
HE2 (Histone Assay Buffer)
HE4 (Biotinylated Substrate, 25 µg/ml)*
HE5 (HMT Standard, 10 µg/ml)*
HE6 (Capture Antibody, 100 µg/ml)*
HE7 (Detection Antibody, 100 µg/ml)*
HE8 (Developing Solution)
HE9 (Stop Solution)
Control Enzyme (150 µg/ml)*
8-Well Assay Strips (with Frame)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
Rugg-Gunn, PJ. et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci USA. 107(24):10783-90. PubMed Abstract
Nakade, K. et. al. (April 2009). JDP2 (Jun Dimerization Protein 2)-deficient mouse embryonic fibroblasts are resistant to replicative senescence. The Journal of Biological Chemistry. 284(16): 10808-17. PubMed Abstract
Contact us about this product :
Our team will respond you as soon as possible !
We have also other products like : EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
52062 Aachen Deutschland
Support Karolina Elandt
Fax: (+49) 241 56 00 47 88
Logistic :0241 40 08 90 86
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
email@example.com | Gentaur
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123
Canada Montreal +15149077481
Ceská republika Praha +420246019719
Finland Helsset +358942419041
Magyarország Budapest +3619980547
US New York+17185132983
WP1006: metapathway biotransformation
WP1124: metapathway biotransformation
WP1212: metapathway biotransformation
WP1251: metapathway biotransformation
WP1286: metapathway biotransformation
WP1581: Histidine metabolism
WP1673: Naphthalene and anthracene degradation
WP1700: Selenoamino acid metabolism
WP1714: Tyrosine metabolism
WP2361: Gastric cancer network 1
WP2366: Butyrate-induced histone acetylation
WP2369: Histone modifications
WP300: Histone modifications
WP702: metapathway biotransformation
WP889: metapathway biotransformation
Related Genes :
 Aberrant histone methylation in the patients with immune thrombocytopenia.
 Aberrant histone modification in peripheral blood B cells from patients with systemic sclerosis.
 Aberrant histone acetylation and methylation levels in woman with endometriosis.
 Abnormal histone modifications in PBMCs from patients with psoriasis vulgaris.
 Abnormal histone modification patterns in lupus CD4+ T cells.
Enter catalog number :