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Index / EpigenTek / EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27 / Product Detail : P-3005-48 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
#P-3005-48 EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
Product name : EpiQuik Histone Methyltransferase Activity_Inhibition Assay Kit H3K27
Catalog number : P-3005-48
Quantity: 48 assays
Supplier name : EpigenTek
Data sheet: Ask more or other datasheet now !
More Details about
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is a convenient set of tools that allows the experimenter to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3-K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition experiment without the need for radioactivity or any special equipment.
WHY CHOOSE THE EPIQUIK™ HISTONE METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT (H3-K27)?
- Very rapid procedure, which can be completed within 3 hours.
- Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
- Specific measurement of activity/inhibition of H3-K27 histone methyltransferases.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Extremely simple, reliable, and consistent assay conditions.
The EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27) is designed for measuring HMTs that specifically target histone H3 at lysine 27. In an assay with this kit, the histone substrate is stably captured on the strip wells through biotin-streptavidin binding. HMT enzymes transfer a methyl group to histone H3 substrate from Adomet to methylate the substrate at lysine 4. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K27, which is directly proportional to enzyme activity, can be quantified through a HRP conjugated secondary antibody-color development system. The HMT activity is then calculated based on the amount of methylated H3-K27 converted by the HMTs.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and H3-K27 specific histone methyltrasferase activity was measured.
HE1 (10X Wash Buffer)
HE2 (Histone Assay Buffer)
HE4 (Biotinylated Substrate, 25 µg/ml)*
HE5 (HMT Standard, 10 µg/ml)*
HE6 (Capture Antibody, 100 µg/ml)*
HE7 (Detection Antibody, 100 µg/ml)*
HE8 (Developing Solution)
HE9 (Stop Solution)
Control Enzyme (150 µg/ml)*
8-Well Assay Strips (with Frame)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
Rugg-Gunn, PJ. et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci USA. 107(24):10783-90. PubMed Abstract
Nakade, K. et. al. (April 2009). JDP2 (Jun Dimerization Protein 2)-deficient mouse embryonic fibroblasts are resistant to replicative senescence. The Journal of Biological Chemistry. 284(16): 10808-17. PubMed Abstract
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WP1006: metapathway biotransformation
WP1124: metapathway biotransformation
WP1212: metapathway biotransformation
WP1251: metapathway biotransformation
WP1286: metapathway biotransformation
WP1581: Histidine metabolism
WP1673: Naphthalene and anthracene degradation
WP1700: Selenoamino acid metabolism
WP1714: Tyrosine metabolism
WP2361: Gastric cancer network 1
WP2366: Butyrate-induced histone acetylation
WP2369: Histone modifications
WP300: Histone modifications
WP702: metapathway biotransformation
WP889: metapathway biotransformation
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