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Index / Genomed / JETSTAR 2.0 Plasmid Midiprep Kit _ 250 / Product Detail : 210250 JETSTAR 2.0 Plasmid Midiprep Kit _ 250
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Sep
28th

#210250 JETSTAR 2.0 Plasmid Midiprep Kit _ 250

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  Price : 1052   EUR
1194   USD
816   GBP
4422   Zloty
140849   JPY
8118   NOK
8698   SEK
1190   CHF

Product name : JETSTAR 2.0 Plasmid Midiprep Kit _ 250

Catalog number : 210250

Quantity: 1 kit

Availability: Yes

Supplier name : Genomed

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More Details about

 
JetStar™ 2.0 Plasmid Purification Kits
The kits contain a unique anion exchange resin, supplied in disposable columns. The Mini, 
Midi and Maxi columns are used under gravity flow conditions without any further instrumentation. JetStar™ 2.0 Plasmid Kits are the ideal tool to efficiently isolate ultrapure plasmid DNA within 45–60 minutes. 
 
Procedure 
 
E. coli cells are harvested, resuspended in Cell Resuspending Buffer (E1) with RNase A, and then lysed with Lysis Buffer (E2). Precipitation Buffer (E3) is added to the lysate and the lysate is clarified by centrifugation. The cleared lysate is passed through a pre-packed anion exchange column. The negatively charged phosphates on the backbone of the DNA interact with the positive charges on the surface of the resin. The temperature, salt concentration, and pH of the solutions influence binding. Under moderate salt conditions, plasmid DNA remains bound to the resin while RNA, proteins, 
carbohydrates and other impurities are washed away with Wash Buffer (E5). The plasmid DNA is eluted under high salt conditions with the Elution Buffer (E6). The eluted DNA is desalted and concentrated with an alcohol precipitation step. The entire protocol can be completed in 1.5–2 hours. 
 
Advantages
 
Using the JetStar™ 2.0 Plasmid Purification Kits to isolate plasmid DNA provides the following advantages: 
• Unique anion exchange resin technology to isolate plasmid DNA negates the need for any organic solvents, cesium chloride (CsCl), or vacuum manifolds 
• High yield of plasmid DNA (see next page) 
• Purified DNA demonstrates improved performance in downstream applications (see next page) 
• Purification of all types and sizes of plasmid DNA, including BAC and cosmid DNA (see pages 23–29), as well as ssM13 DNAs, λ-DNA, and plasmid DNA from fungi (protocols available at www.genomed-dna.com) 
• Minimal contamination with RNA 
 

 

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