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Index / Celltrend / EIA for Quantitative Determination of Thaumatin / Product Detail : 30100 EIA for Quantitative Determination of Thaumatin
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#30100 EIA for Quantitative Determination of Thaumatin

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  Price : 885   EUR
1005   USD
687   GBP
3721   Zloty
118519   JPY
6831   NOK
7319   SEK
1001   CHF

Product name : EIA for Quantitative Determination of Thaumatin

Catalog number : 30100

Quantity: 1 x 96

Availability: Yes

Supplier name : Celltrend

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About this Product :

EIA for Quantitative Determination of Thaumatin antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of EIA for Quantitative Determination of Thaumatin.
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More Details about

Introduction
The thaumatins is a mixture of proteins isolated from
the katemfe fruit (Thaumatococcus daniellii Bennett).
Some of the proteins in the thaumatin family are
natural sweeteners roughly 2000 times more potent
than sugar. Thaumatin has been approved as a
sweetener in the European Union (E957), Israel, and
Japan. In the United States, it is a Generally
Recognized as Safe flavoring agent (FEMA GRAS
3732). The CellTrend Thaumatin-ELISA is designed
for the quantitative determination of Thaumatin in
complex samples.
 
 
Principle of the assay
The determination of Thaumatin is carried out as
direct sandwich ELISA. An antibody specific for
Thaumatin has been pre-coated onto a microplate.
Standards and samples are pipetted into the wells
and any Thaumatin present is bound.. After washing
away any unbound substances, an enzyme-linked
antibody is added. Following a wash, a substrate
solution is added to the wells and color develops in
proportion to the amount of antibody conjugate. The
absorption at 450 nm is proportional to the
Thaumatin concentration
 
Performance Characteristics
Standard curve:
7 Standards between 0,8 ng/ml and 50 ng/ml
Sensitivity:
at a 1:100 sample dilution 80 ng/ml
Sample materials::
Food after extraction
Intraassay precision (CV)::
(n=10)
at 6 ng/ml: 6,2%
Cross reactivity:
Acesulfamat <0,1%
Aspartam <0,1%
Cyclamat <0,1%
Saccharin <0,1%
Saccharose <0,1%
 
Assay Procedure
Preparing of samples / extraction                                                                     < 1 h
Incubation of samples/standards                       100 μl/well                          2 h
Wash
Incubation of detection antibody                           100 μl/well                         2 h
Wash
Substrate incubation                                                100 μl/well                       15 min
Add stop solution                                                      100 μl/well
Read at 450 nm

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