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Index / Bioassays / EnzyChrom™ Sucrose Assay Kit / Product Detail : ESUC-100 EnzyChrom™ Sucrose Assay Kit
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Dec
10th

#ESUC-100 EnzyChrom™ Sucrose Assay Kit

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  Price : 337   EUR
382   USD
261   GBP
1415   Zloty
45089   JPY
2598   NOK
2784   SEK
381   CHF

Product name : EnzyChrom™ Sucrose Assay Kit

Catalog number : ESUC-100

Quantity: 100 Tests

Availability: Yes

Supplier name : Bioassays

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More Details about

For quantitative determination of sucrose.
Method: OD565nm.
Samples: serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc.
Species: all.
Procedure: 70 min.
Size: 100 tests.
Detection limit: 17 μM.

DESCRIPTION
SUCROSE (C12H22O11) is a disaccharide of glucose and fructose with an
α-1,2-glycosidic linkage. It is the most common food sweetener and the
most important sugar in plants. In mammals, sucrose is readily digested
in the stomach into glucose and fructose, which are rapidly absorbed into
the bloodstream in the small intestine. Simple, direct and high-throughput
assays for measuring sucrose concentrations find wide applications.
BioAssay Systems' improved assay uses invertase to digest sucrose into
fructose and glucose. The resulting fructose is then quantified using our
fructose assay reagent. The measured color intensity at 565nm is
directly proportional to the sucrose concentration in the sample.
KEY FEATURES
No interference by glucose.
Use 20 μL samples. Linear detection range: 17 to 2000 μM sucrose.
APPLICATIONS
Assays: sucrose in biological samples (e.g. serum, plasma, urine,
saliva, milk, culture medium), food, juice, beverage and other agricultural
products.
Drug Discovery/Pharmacology: effects of drugs on sucrose metabolism.
KIT CONTENTS
Assay Buffer: 10 mL Invertase: 120 μL
PMS Solution: 1.5 mL Enzyme: 120 μL
MTT Solution: 1.5 mL Standard: 400 μL 40 mM Sucrose
Storage conditions. The kit is shipped on ice. Store all components at
-20°C. Shelf life of three months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.

MATERIALS REQUIRED, BUT NOT PROVIDED
Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well
plates, optical density plate reader.

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