GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908,

Index / Bioassays / QuantiChrom™ â_Glucosidase Assay Kit / Product Detail : DBGD-100 QuantiChrom™ â_Glucosidase Assay Kit
Related keywords:


#DBGD-100 QuantiChrom™ â_Glucosidase Assay Kit

Ask technical file .

  Price : 493   EUR
559   USD
382   GBP
2071   Zloty
65987   JPY
3803   NOK
4075   SEK
557   CHF

Product name : QuantiChrom™ â_Glucosidase Assay Kit

Catalog number : DBGD-100

Quantity: 100 Tests

Availability: Yes

Supplier name : Bioassays

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

QuantiChrom™ â_Glucosidase Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ â_Glucosidase Assay Kit.

More Details about

b-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds
linking two glucose or glucose-substituted molecules (i.e., the disaccharide
cellobiose). b-Glucosidases are required by organisms (some fungi,
bacteria, termites) for consumption of cellulose. Lysozyme is also a b-
glucosidase and is present in tears to prevent bacterial infection of the eye.
In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase)
has been related to Gaucher's disease and Parkinson's
Simple, direct and automation-ready procedures for measuring b-
glucosidase activity are becoming popular in Research and Drug Discovery.
BioAssay Systems' QuantiChromTM b-Glucosidase Assay Kit is designed to
measure b-glucosidase activity directly in biological samples without
pretreatment. The improved method utilizes p-nitrophenyl-b-Dglucopyranoside
that is hydrolyzed specifically by b-glucosidase into a
yellow colored product (maximal absorbance at 405nm). The rate of the
reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection
limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple “mix-and-measure”
procedure allows reliable quantitation of b-glucosidase activity within 20
Robust and amenable to HTS. All reagents are compatible with highthroughput
liquid handling instruments.
Direct Assays: b-glucosidase activity in biological samples.
Characterization and Quality Control for b-glucosidase production.
Drug Discovery: high-throughput screen for b-glucosidase modulators.
KIT CONTENTS (100 tests in 96-well plates)
Assay Buffer: 24 mL (pH 7.0)
b-NPG Substrate: 1 mL
Calibrator: 10 mL (equivalent to 250 U/L)
Storage conditions. The kit is shipped at room temperature. Store all
components at -20°C. Shelf life of 6 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents. Please
refer to Material Safety Data Sheet for detailed information.
PROCEDURES. This assay is based on a kinetic reaction. Use of a
multi-channel pipettor is recommended. Addition of Working Reagent to
samples should be quick and mixing should be brief but thorough. Assays
can be executed at room temperature or 37°C.
Reagent preparation: equilibrate reagents to room temperature. The
Working Solution is prepared by mixing for each 96-well assay, 200 μL
Assay Buffer and 8 μL b-NPG substrate (final 1.0 mM). Fresh reconstitution
is recommended, although the Working Solution is stable for at least one
day at room temperature.
Sample preparation: enzyme samples can be in 50 mM phosphate (pH 7.0)
buffer or in any other suitable enzyme buffer. The following chemicals are
known to affect the enzyme activity and should be avoided. SH-containing
reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+,
Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, EDTA and Tris.
Procedure using 96-well plate:
1. Transfer 20 μL distilled water (H2O) to two wells of a clear bottom 96-well
plate. Add 200 μL H2O to one of these wells and 200 μL Calibrator to the
other well (total volume 220 μL).
Transfer 20 μL samples into other wells. Transfer 200 μL Working
Reagent to the sample wells only. The final reaction volume in the
sample wells is 220 μL. Tap plate briefly to mix.
2. Read OD405nm (t = 0), and again after 20 min (t = 20 min) on a plate reader.
4. Calculation: b-glucosidase activity of the sample (U/L) is
OD20 – OD0
b-Glucosidase Activity × 250 (U/L)
OD20 and OD0 are OD405nm values of sample at 20 and 0 min,
respectively. ODCALIBRATOR and ODH2O are OD405nm values of Calibrator
and H2O at 20 min.
Unit definition: one unit of enzyme catalyzes the hydrolysis of 1 μmole of
substrate per min at pH 7.0.
Pipeting devices and accessories (e.g. multi-channel pipettor). Clear
bottom 96-well plates (e.g. Corning Costar) and plate reader.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Bioassays \ QuantiChrom™_â_Glucosidase_Assay_Kit \ DBGD_100
Reload Image


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45 | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411 | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925 | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897 | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

tel:0911876558 | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48       | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94 | Gentaur