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Index / Bioassays / QuantiChrom™ Heme Assay Kit / Product Detail : DIHM-250 QuantiChrom™ Heme Assay Kit
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Aug
26th

#DIHM-250 QuantiChrom™ Heme Assay Kit

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  Price : 308   EUR
349   USD
239   GBP
1294   Zloty
41224   JPY
2376   NOK
2545   SEK
348   CHF

Product name : QuantiChrom™ Heme Assay Kit

Catalog number : DIHM-250

Quantity: 250 Tests

Availability: Yes

Supplier name : Bioassays

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About this Product :

QuantiChrom™ Heme Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Heme Assay Kit.
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More Details about

DESCRIPTION
Heme is one important member of the porphyrin family. It is synthesized
in both mitochondria and cytoplasm, and is a key prosthetic group for
various essential proteins such as hemoglobin, cytochromes, catalases
and peroxidases. Heme determination is widely practiced by researchers
of various blood diseases.
Simple, direct and automation-ready procedures for measuring heme
concentration are becoming popular in Research and Drug Discovery.
BioAssay Systems' QuantiChromTM Heme Assay Kit is based on an
improved aqueous alkaline solution method, in which the heme is
converted into a uniform colored form. The intensity of color, measured
at 400 nm, is directly proportional to the heme concentration in the
sample. The optimized formulation substantially reduces interference by
substances in the raw samples and exhibits high sensitivity.


APPLICATIONS
Direct Assays: total heme in blood, serum, plasma, urine, hemecarrying
enzymes.
Pharmacology: effects of drugs on heme metabolism.
Drug Discovery: HTS for drugs that modulate heme levels.


KEY FEATURES
Sensitive and accurate. Linear detection range 0.6 – 125 μM heme in
96-well plate assay.
Simple and high-throughput. The “mix-and-read” procedure involves
addition of a single working reagent and reading the optical density. Can
be readily automated as a high-throughput assay in 96-well plates for
thousands of samples per day.
Safety. Reagents are non-toxic.
Versatility. Assays can be executed in 96-well plate or cuvet.


KIT CONTENTS (250 tests in 96-well plates)
Reagent: 50 mL
Calibrator: 10 mL (equivalent to 62.5 μM heme)
Storage conditions. The kit is shipped room temperature. Store reagent
and Calibrator at 4°C. Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.

MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories.
Procedure using 96-well plate:
Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.
Procedure using cuvette:
Cuvets and spectrophotometer.

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