GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Bioassays / QuantiChrom™ Copper Assay Kit / Product Detail : DICU-250 QuantiChrom™ Copper Assay Kit
Related keywords:

Dec
8th

#DICU-250 QuantiChrom™ Copper Assay Kit

Ask technical file .

  Price : 524   EUR
595   USD
406   GBP
2202   Zloty
70138   JPY
4042   NOK
4331   SEK
592   CHF

Product name : QuantiChrom™ Copper Assay Kit

Catalog number : DICU-250

Quantity: 250 Tests

Availability: Yes

Supplier name : Bioassays

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

QuantiChrom™ Copper Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ Copper Assay Kit.
http://mybiofast.com/ver.php?search=anti-&submit=Search

More Details about

DESCRIPTION
Copper is an essential trace element. Copper-containing enzymes play
important roles in iron and catecholamine metabolism, free radical
scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper
is mainly present in caeruloplasmin in the liver. Low levels of copper have
been associated with mental retardation, depigmentation, anaemia,
hypotonia and scorbutic changes in bone. Levels of copper are key
diagnostic indicator of diseases such as Wilson's disease, microcytic
hypochromic anaemia and bone disease due to reduced collagen synthesis.
Simple, direct and automation-ready procedures for measuring copper
concentrations find wide applications in research, drug discovery and
environmental monitoring. BioAssay Systems' copper assay kit is
designed to measure copper with no or minimal sample treatment. The
improved method utilizes a chromogen that forms a colored complex
specifically with copper ions. The intensity of the color, measured at
359nm, is directly proportional to copper concentration in the sample.
The optimized formulation substantially reduces interference by
substances in the raw samples.
KEY FEATURES
Sensitive and accurate. Linear detection range 7 μg/dL (1.0 μM) to 300
μg/dL (47 μM) copper in 96-well plate assay.
Simple and high-throughput. The simple procedure can be readily
automated as a high-throughput assay in 96-well plates for thousands of
samples per day.
Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
APPLICATIONS
Direct Assays: biological, environmental, food and beverage samples.
Drug Discovery/Pharmacology: effects of drugs on Cu metabolism.
KIT CONTENTS (250 tests in 96-well plates)
Reagent A: 10 mL Reagent B: 1.5 mL Reagent C: 40 mL
Copper Standard: 1 mL 1.5 mg/dL Cu2+
Storage conditions. The kit is shipped at room temperature. Store all
reagents at 4 °C. Shelf life: 12 months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
PROCEDURES
Note: metal chelators (e.g. EDTA) interfere with this assay and should be
avoided in sample preparation.
Procedure using 96-well plate:
1. Standards: transfer 100 μL dH2O into one Eppendorf tube labeled
“Blank”. Into another tube labeled “Standard”, mix 20 μL 1.5 mg/dL
Standard and 80 μL dH2O (final 300 μg/dL Cu2+).
Samples: transfer 100 μL samples into separate tubes.
Add 35 μL Reagent A (trichloroacetic acid) to each tube and mix by
vortexing. If samples contain protein (e.g. serum/plasma), precipitates
form. Centrifuge tubes for 2 min at 14,000 rpm and use clear
supernatant for assay. For samples that do not contain protein, the
mixture remains clear and centrifugation is not necessary.
Transfer 100 μL Blank, Standard and Sample into separate wells of a
clear flat-bottom 96-well plate.
2. For each assay well, prepare Working Reagent by mixing 5 μL Reagent
B and 150 μL Reagent C. Transfer 150 μL Working Reagent to each
well and tap plate to mix thoroughly.
3. Incubate 5 min at room temperature and read optical density at 356-
362nm (peak absorbance at 359nm).
Note: if sample OD values are higher than the OD value for the 300μg/dL
Standard, dilute sample in dH2O and repeat assay. Multiply the results by the
dilution factor.
Procedure using cuvette:
Prepare standards and samples as for 96-well assay procedure.
1. Transfer 400 μL Standards and Samples into separate cuvets.
2. Add 600 μL Working Reagent. Mix by pipetting.
3. Incubate 5 min at room temperature and read optical density at
356-362nm (peak absorbance at 359nm).
CALCULATION
The copper concentration of Sample is calculated as
=
ODSAMPLE – ODBLANK
ODSTANDARD – ODBLANK
× 300 (μg/dL)
ODSAMPLE, ODBLANK and ODSTANDARD are optical density values of the
Sample, Blank and the 300 μg/dL Standard, respectively.
Conversions: 100 μg/dL Cu equals 15.5 μM, 0.0001% or 1 ppm.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories. For 96-well plate assays: clear
flat-bottom 96-well plates and plate reader. For cuvet assays:
spectrophotometer and cuvets for measuring OD at 356-362nm.
GENERAL CONDSIDERATIONS
For scarce samples (e.g. mice serum or plasma), mix sample with
dH2O to a total of 100 μL, e.g. 50 μL serum + 50 μL dH2O. Multiply
the results by the dilution factor (2 fold).
EXAMPLES
Human serum, rat plasma, rat serum, and bovine serum were
assayed in duplicate using the 96-well plate assay protocol. The
copper concentrations were 97 ± 1, 104 ± 1, 101 ± 2, 78 ± 1 μg/dL,
respectively.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Bioassays \ QuantiChrom™_Copper_Assay_Kit \ DICU_250
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur