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Index / Bioassays / CellQuanti_Blue™ Cell Viability Assay Kits / Product Detail : CQBL-10K CellQuanti_Blue™ Cell Viability Assay Kits
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Dec
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#CQBL-10K CellQuanti_Blue™ Cell Viability Assay Kits

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  Price : 355   EUR
403   USD
275   GBP
1492   Zloty
47522   JPY
2739   NOK
2934   SEK
401   CHF

Product name : CellQuanti_Blue™ Cell Viability Assay Kits

Catalog number : CQBL-10K

Quantity: 10000 Tests

Availability: Yes

Supplier name : Bioassays

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About this Product :

CellQuanti_Blue™ Cell Viability Assay Kits antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of CellQuanti_Blue™ Cell Viability Assay Kits.
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More Details about

DESCRIPTION
The study of cell proliferation and cell viability requires the accurate
quantification of the number of viable cells in a cell culture. Therefore,
assays for calculating cell viability are necessary for optimizing cell culture
conditions, evaluating cell growth factors and nutrients, discovering novel
antibiotics and anti-cancer drugs, evaluating toxic effects of environmental
pollutants and cell mediated toxicity and studying programmed cell death
(apoptosis).
The CellQuanti-MTTTM assay kit provides a convenient, sensitive,
quantitative and reliable assay for determining the number of viable cells in
a given culture. This homogeneous colorimetric assay is based on the
conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan,
a purple dye. This cellular reduction reaction involves the pyridine
nucleotide cofactors NADH/NADPH and is only catalyzed by living cells.
The formazan product has a low aqueous solubility and is present as purple
crystals. Dissolving the resulting formazan with a solubilization buffer
permits the convenient quantification of product formation. The intensity of
the product color, measured at 550 - 620 nm, is directly proportional to the
number of living cells in the culture. Reagents in the kit have been carefully
formulated and optimized for sensitivity, assay robustness and automation.
KEY FEATURES
Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
Sensitive and accurate. As low as 950 cells can be accurately quantified.
Fast. High-throughput assay using 96-well plates allows simultaneous
processing tens of thousands of samples per day.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No
wash and reagent transfer steps are involved.
Robust and amenable to HTS. Z’ factors of 0.5 and above are observed.
Can be readily automated with HTS liquid handling systems.
APPLICATIONS
Cell Proliferation: effects of cytokines, growth factor, nutrients.
Cytotoxicity and Apoptosis: evaluation of toxic compounds, anti-cancer
antibodies, toxins, environmental pollutants etc.
Drug Discovery: high-throughput screen for toxic and anticancer drugs

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