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#K3003-1 Cortisol Enzyme Immunoassay kit _ 1 Strip plate
Product name : Cortisol Enzyme Immunoassay kit _ 1 Strip plate
Catalog number : K3003-1
Quantity: 96 rxns
Supplier name : BBridge
Data sheet: Ask more or other datasheet now !
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Cortisol Enzyme Immunoassay kit - 1 Strip plate
The Cortisol Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts and in saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in serum and plasma and free cortisol in urine. A cortisol standard is provided to generate a standard curve for the assay.
Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After a 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is read at 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers.
Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the stress hormone as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer, depression,and schizophrenia. It is already known that abnormal levels of cortisol are involved in Cushings Syndrome and Addisions disease.
Dried Fecal Extracts and Saliva, Urine, Serum, EDTA and Heparin Plasma and Tissue Culture Media (TCM) samples have been validated with this assay.
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WP142: mRNA processing
WP1493: Carbon assimilation C4 pathway
WP1815: Factors involved in megakaryocyte development and platelet production
WP1819: Formation of Platelet plug
WP1881: Platelet activation triggers
WP1882: Platelet Activation
WP1883: Platelet Adhesion to exposed collagen
WP1884: Platelet Aggregation (Plug Formation)
WP1885: Platelet homeostasis
WP1903: Response to elevated platelet cytosolic Ca2+
WP1970: Glycoprotein VI platelet signaling
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP2349: vitamin B3 (niacin), NAD and NADP biosynthesis pathway
WP508: Biosynthesis of Aldosterone and Cortisol
WP626: Abscisic Acid Biosynthesis
Related Genes :
[NR3C1 GRL] Glucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group C member 1)
[PTPN11 PTP2C SHPTP2] Tyrosine-protein phosphatase non-receptor type 11 (EC 22.214.171.124) (Protein-tyrosine phosphatase 1D) (PTP-1D) (Protein-tyrosine phosphatase 2C) (PTP-2C) (SH-PTP2) (SHP-2) (Shp2) (SH-PTP3)
[BMP2 BMP2A] Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A)
[PRKACA PKACA] cAMP-dependent protein kinase catalytic subunit alpha (PKA C-alpha) (EC 126.96.36.199)
[GHRL MTLRP UNQ524/PRO1066] Appetite-regulating hormone (Growth hormone secretagogue) (Growth hormone-releasing peptide) (Motilin-related peptide) (Protein M46) [Cleaved into: Ghrelin-27; Ghrelin-28 (Ghrelin); Obestatin]
[Bmp2 Bmp-2] Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A)
[CYP11B1 S11BH] Cytochrome P450 11B1, mitochondrial (CYPXIB1) (Cytochrome P-450c11) (Cytochrome P450C11) (Steroid 11-beta-hydroxylase) (EC 188.8.131.52)
[Wnt4 Wnt-4] Protein Wnt-4
[GNAS GNAS1 GSP] Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein)
[Ptpn11] Tyrosine-protein phosphatase non-receptor type 11 (EC 184.108.40.206) (Protein-tyrosine phosphatase SYP) (SH-PTP2) (SHP-2) (Shp2)
[NR3C2 MCR MLR] Mineralocorticoid receptor (MR) (Nuclear receptor subfamily 3 group C member 2)
[HPGD PGDH1 SDR36C1] 15-hydroxyprostaglandin dehydrogenase [NAD(+)] (15-PGDH) (EC 220.127.116.11) (Prostaglandin dehydrogenase 1) (Short chain dehydrogenase/reductase family 36C member 1)
[SERPINA6 CBG] Corticosteroid-binding globulin (CBG) (Serpin A6) (Transcortin)
[CYP21A2 CYP21 CYP21B] Steroid 21-hydroxylase (EC 18.104.22.168) (21-OHase) (Cytochrome P-450c21) (Cytochrome P450 21) (Cytochrome P450 XXI) (Cytochrome P450-C21) (Cytochrome P450-C21B)
[HSD11B2 HSD11K SDR9C3] Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC 1.1.1.-) (11-beta-hydroxysteroid dehydrogenase type 2) (11-DH2) (11-beta-HSD2) (11-beta-hydroxysteroid dehydrogenase type II) (11-HSD type II) (11-beta-HSD type II) (NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (11-beta-HSD) (Short chain dehydrogenase/reductase family 9C member 3)
[SLIT2 SLIL3] Slit homolog 2 protein (Slit-2) [Cleaved into: Slit homolog 2 protein N-product; Slit homolog 2 protein C-product]
[CAD] CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase (EC 22.214.171.124); Aspartate carbamoyltransferase (EC 126.96.36.199); Dihydroorotase (EC 188.8.131.52)]
[WNT4 UNQ426/PRO864] Protein Wnt-4
[DGKQ DAGK4] Diacylglycerol kinase theta (DAG kinase theta) (EC 184.108.40.206) (Diglyceride kinase theta) (DGK-theta)
[nr3c2 mlr] Mineralocorticoid receptor (MR) (Nuclear receptor subfamily 3 group C member 2) (Fragment)
[NNT] NAD(P) transhydrogenase, mitochondrial (EC 220.127.116.11) (Nicotinamide nucleotide transhydrogenase) (Pyridine nucleotide transhydrogenase)
[Crh] Corticoliberin (Corticotropin-releasing factor) (CRF) (Corticotropin-releasing hormone)
[REST NRSF XBR] RE1-silencing transcription factor (Neural-restrictive silencer factor) (X2 box repressor)
[HSD11B1 HSD11 HSD11L SDR26C1] Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC 18.104.22.168) (11-beta-hydroxysteroid dehydrogenase 1) (11-DH) (11-beta-HSD1) (Short chain dehydrogenase/reductase family 26C member 1)
[Ptpn11] Tyrosine-protein phosphatase non-receptor type 11 (EC 22.214.171.124) (Protein-tyrosine phosphatase 1D) (PTP-1D) (Protein-tyrosine phosphatase SYP) (SH-PTP2) (SHP-2) (Shp2)
[CACNA1H] Voltage-dependent T-type calcium channel subunit alpha-1H (Low-voltage-activated calcium channel alpha1 3.2 subunit) (Voltage-gated calcium channel subunit alpha Cav3.2)
[POMC] Pro-opiomelanocortin (POMC) (Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma (Gamma-MSH); Potential peptide; Corticotropin (Adrenocorticotropic hormone) (ACTH); Melanotropin alpha (Alpha-MSH); Corticotropin-like intermediary peptide (CLIP); Lipotropin beta (Beta-LPH); Lipotropin gamma (Gamma-LPH); Melanotropin beta (Beta-MSH); Beta-endorphin; Met-enkephalin]
[Ghrl] Appetite-regulating hormone (Growth hormone secretagogue) (Growth hormone-releasing peptide) (Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin-23; Obestatin-13]
[Cyp1b1] Cytochrome P450 1B1 (EC 126.96.36.199) (CYPIB1) (Cytochrome P450RAP)
[GNAS GNAS1] Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (Adenylate cyclase-stimulating G alpha protein) (Extra large alphas protein) (XLalphas)
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