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Index / BBridge / Leptin ELISA Kit, Rat / Product Detail : 297-57601 Leptin ELISA Kit, Rat
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Dec
6th

#297-57601 Leptin ELISA Kit, Rat

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  Price : 1505   EUR
1708   USD
1167   GBP
6323   Zloty
201397   JPY
11607   NOK
12437   SEK
1702   CHF

Product name : Leptin ELISA Kit, Rat

Catalog number : 297-57601

Quantity: 96 tests

Availability: Yes

Supplier name : BBridge

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About this Product :

Leptin ELISA Kit, Rat
http://antibody-antibodies.com/anti-elisa/ELISA.html
EIA kit description
Leptin ELISA Kit, Rat is an ELISA kit which is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from dilution of the samples, through the incubation to the blocking and washing.

More Details about

Leptin, secreted from fatty cells, is known as a hormone controlling body fat by suppresssion of eating and increase of energy metabolism. It is reported, however, that expression of leptin gene in fatty tissue and concentration of leptin in blood are at high levels in obese people and model animals of obesity. Samples for serum and plasma : 312.5 ~ 20,000 pg/mL Samples for others than serum and plasma : 78.1 ~ 5,000 pg/mL Specific to rat Leptin, but only slightly cross-reactive to human Leptin.

Principle of the assay
This kit is a sandwich-format of ELISA with two antibodies specific to rat leptin. Monoclonal antibody to leptin is coated on the microplate well, which captures leptin in sample. Following reaction with HRPconjugated rabbit anti rat leptin antibody, forms HRP labeled antigenantibody complex on the well, which reports the signal of leptin amount in the sample by chromogenic reaction associated with
catalysis of hydrogen peroxide.


Dynamic range :
• Samples for serum and plasma : 312.5 ~ 20,000 pg/mL
• Samples for others than serum and plasma : 78.1 ~ 5,000 pg/mL


Cross-reactivity
Specific to rat Leptin, but only slightly cross-reactive to human Leptin.

Kit Contents (96 tests)
1. Antibody-coated Microtiter Plate 1 plate
(Anti Rat Leptin, monoclonal antibody)
2. Rat Leptin Standard 20 ng
3. HRP-conjugated Antibody, rabbit 6 mL
4. Chromogen (OPD Tablet) 2 tablets
5. Chromogen Diluent Solution 24 mL
6. Wash Stock Solution (20 x) 50 mL
7. Buffer A 20 mL
8. Buffer B 20 mL
9. Stop Solution (1mol/L H2SO4) 12 mL
10. Adhesive Plate Cover 2 covers

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