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Index / Trevigen / Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED / Product Detail : 3400-PC-100 Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED
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#3400-PC-100 Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED

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Product name : Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED

Catalog number : 3400-PC-100

Quantity: 100 µl

Availability: Yes

Supplier name : Trevigen

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About this Product :

Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED.
http://mybiofast.com/ver.php?search=anti-&submit=Search

Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED mus musculus murine Rabbit anti_Mouse Laminin I Polyclonal Antibody *DISCONTINUED detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

Description: This highly purified preparation of mouse laminin I increases cell adhesion, migration, growth, and differentiation. It is composed of α1β1γ1 chains with a total Mr of 800,000 and is used for coating culture dishes.
Source: Murine Engelbreth-Holm-Swarm (EHS) tumor.
Storage Buffer: Dulbecco’s Modified Eagle’s Medium with 10 μg/ml gentamycin sulfate.
Storage: Store at -20°C in a manual defrost freezer or -80°C.
Purity: Purity >90% by SDS-PAGE.
Specifications:
Functional Assays:
• Supports the attachment (at least 50%) of MG63 human osteosarcoma cells to a laminincoated plate at a coating solution concentration of 20 μg/ml.
Sterility Testing:
• No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP XXIV, Chapter 71 sterility testing.
• PathClear®: Negative by PCR test for: mycoplasma, 17 bacterial and virus strains
typically included in mouse antibody production (MAP) testing, plus 13 additional murine
infectious agents including LDEV, for a total of 31 organisms and viruses.
• Endotoxin concentrations < 20 EU/ml by LAL assay.


Coating procedure:
The recommended working concentration is 0.05-10 μg/cm2 of growth surface (0.05 -10 μg/ml) depending on cell type.
A. Thaw stock solution on ice for several hours. In a laminar flow hood, dilute appropriately with cold serum-free, cell culture medium. Mix and transfer to the wells of tissue culture plates. Spread the solution to completely cover the bottom of the wells.
B. The following table is a guide for the suggested volumes required per well:
Plate type/ Volume Laminin per Well
6 wells (or 35 mm dish) 2 ml
24 wells 1 ml
48 wells 200 μl
96 wells 100 μl
C. Incubate the plates at 37°C from 4 hours to overnight. In the laminar flow hood, remove excess liquid from the wells of the tissue culture plate.
D. Rinse the wells once with cell culture medium and then add your cells.

 

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