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Index / Biotium / FURA_2, PENTAAMMONIUM SALT / Product Detail : 50030 FURA_2, PENTAAMMONIUM SALT
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#50030 FURA_2, PENTAAMMONIUM SALT

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  Price : 237   EUR
269   USD
184   GBP
997   Zloty
31777   JPY
1831   NOK
1962   SEK
268   CHF

Product name : FURA_2, PENTAAMMONIUM SALT

Catalog number : 50030

Quantity: 1

Availability: Yes

Supplier name : Biotium

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More Details about

DESCRIPTION: HDAC assay developer is an essential reagent for signal generation in
HDAC or sirtuin assays performed with any of BPS Bioscience’s HDAC fluorimetric
substrates (e.g. Cat. #50032). An appropriate fluorimetric substrate, comprising an
acetylated lysine side chain, is first incubated with a sample containing HDAC activity
(nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the
HDAC assay developer produces a fluorophore. Trichostatin A is included as an
‘inhibitor stop’ for class I and II HDACs.

APPLICATIONS: Study of HDAC or sirtuin kinetics, regulation and inhibitor sensitivity.
Ideal for drug discovery and HTS applications and cell-based HDAC assays.

SUPPLIED AS: A 2x concentrate.

QUANTITY: 6 ml

STORAGE: -70°C. Store, long term, only as the 2x concentrate, at -70°C. Concentrate is
stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and
thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid fingerrubbing
for thawing). Frozen storage of smaller aliquots is recommended.

REFERENCES:
1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99 8921
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302 175
3. S. Milutinovic et al. J. Biol. Chem. 2002 277 20974

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