GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Biotium / FURA_2,AM ESTER,100UG / Product Detail : 50033 FURA_2,AM ESTER,100UG
Related keywords:

Dec
5th

#50033 FURA_2,AM ESTER,100UG

Ask technical file .

  Price : 296   EUR
336   USD
229   GBP
1244   Zloty
39649   JPY
2285   NOK
2448   SEK
335   CHF

Product name : FURA_2,AM ESTER,100UG

Catalog number : 50033

Quantity: 10

Availability: Yes

Supplier name : Biotium

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

DESCRIPTION: The Fluorogenic HDAC Assay Kit is a complete assay system
designed to measure histone deacetylase (HDAC) class 1 activity for screening and
profiling applications. It comes in a convenient 96-well format, with all the reagents
necessary for 100 fluorescent HDAC activity measurements. In addition, the kit
includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,
for use as a positive and negative control. The Fluorogenic HDAC Assay
Kit is based on a unique fluorogenic substrate and developer combination. This assay
method eliminates dealing with the radioactivity, extraction, and chromatography aspects
of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed
to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing
an acetylated lysine side chain, is incubated with purified HDAC enzyme.
The deacetylation sensitizes the substrate so subsequent treatment with the Lysine
Developer produces a fluorophore that can then be measured using a fluorescence reader.

HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from
acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are
attached to a peptide containing acetyllysine. Attachment to the peptide quenches the
fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is
mixed with a development solution that is specific for nonacetylated lysines. If the acetyl
group has been removed from the lysine by the HDAC, this solution will release the dye
allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity.

 

COMPONENTS:
Fluorogenic HDAC Assay Kit Components

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

REFERENCES:
1. A. Ito et al. (2001) EMBO J. 20 1331.
2. N.A. Barlev et al. (2001) Mol. Cell 8 1243.
3. A. Ito et al. (2002) EMBO J. 21 6236.

APPLICATION REFERENCES:
1. Huber, K., et. al, J. Biol. Chem. 286(25): 22211 – 22218 (2011).
2. Chun, P., et. al., Bull. Korean Chem. Soc. 32(6): 1891 – 1896 (2011).

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Biotium \ FURA_2,AM_ESTER,100UG \ 50033
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur