GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Biovis / Procathepsin K, human recombinant / Product Detail : 1026-10 Procathepsin K, human recombinant
Related keywords:

Dec
9th

#1026-10 Procathepsin K, human recombinant

Ask technical file .

  Price : 307   EUR
348   USD
238   GBP
1289   Zloty
41081   JPY
2367   NOK
2537   SEK
347   CHF

Product name : Procathepsin K, human recombinant

Catalog number : 1026-10

Quantity: 10 μg

Availability: Yes

Supplier name : Biovis

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

Procathepsin K, human recombinant recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the Procathepsin K, human recombinant.
http://mybiofast.com/ver.php?search=rec.&submit=Search

Procathepsin K, human recombinant Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Procathepsin K, human recombinant.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

AMOUNT: 10 μg
SOURCE: E. coli (amino acid 19 – 329). Methionine was introduced at amino acid number 18 to create a new amino-terminal sequence MEEIL. Genbank: S79895; Swiss Prot: P43235
PURITY: >95% by SDS-PAGE analyses
FORM: Liquid (25 mM Tris pH 8.0, 500 mM NaCl)
CONCENTRATION: 200μg/ml
SPECFIC ACTIVITY: After auto-catalytical activation at pH 4.0 the specific activity is >1000 mU/mg (international unit 1μmole/min/mg)


ACTIVATION:
Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT.
Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine’s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups (Nishimura et al., 1975).This reversible reaction arrests the autoproteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution.


STORAGE CONDITIONS:
Procathepsin K can be aliquoted and stored at -80°C. Repeated freeze-thaw cycles must be avoided.


DESCRIPTION:
Cathepsin K is a member of the papain cysteine proteinase family and has been identified as the predominant proteinase responsible for the resorption of the bone matrix. The enzyme cleaves proteins such as collagen type I, collagen type II and osteonectin and therefore plays a role in bone remodeling and resorption in diseases such as osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.


INHIBITORS:
leupeptin (IC50 ; 70 nM), E-64 (IC50 ; 5nM), and cystatin. Minimal effect shows pepstatin and phenylmethylsulfonyl fluoride, inhibitors of aspartyl and serine proteases, respectively. No inhibition was observed by addition of EDTA or phenanthroline, classical inhibitors of metalloproteases.


IMAGE:


RELATED PRODUCTS:
Cathepsin K Antibody (Cat# 3368-100)
Cathepsin K Activity Assay Kit (Cat# K141-100)
Cathepsin K Antibody (Cat#3588-100)
Cathepsin K Blocking Peptide (Cat# 3368BP-50)
Cathepsin K Blocking Peptide (Cat#3588BP-50)

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Biovis \ Procathepsin_K,_human_recombinant \ 1026_10
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur