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#1135-5 Cathepsin L, human recombinant

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Product name : Cathepsin L, human recombinant

Catalog number : 1135-5

Quantity: 5 μg

Availability: Yes

Supplier name : Biovis

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BACKGROUND DESCRIPTION:
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer.

SOURCE:
The recombinant Cathepsin L was produced from E. coli encoding human cDNA sequence from 188 to 1135 of gene accession number M20496 with 6XHis-tag on its N-terminus. The protein was purified by several chromatographic steps.

FORM: Lyophilized from 50 mM HEPES buffer, pH 4.0.
PURITY: >90% by SDS-PAGE
RECONSTITUTION: With dH2O to 0.1 – 1 mg/ml.

STORAGE CONDITIONS:
Store at –70°C, stable for at least 1 year as supplied. Avoid freeze/thaw cycles.

APPLICATIONS:
Recombinant Cathepsin L is useful in inhibitor screening, kinetic studies and cleavage of substrates in vitro or in tissue culture.

SPECIFIC ACTIVITY:
6000 units/mg protein. One unit will hydrolyze one nmol Z-Phe-Arg-AFC substrate per minute at 37°C in 400 mM Sodium Acetate, pH 5.5, with 4 mM EDTA and 10 mM DTT.

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