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Index / Genomed / JETSTAR 2.0 Plasmid Maxiprep Kit _ 100 / Product Detail : 220 100 JETSTAR 2.0 Plasmid Maxiprep Kit _ 100
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Dec
9th

#220 100 JETSTAR 2.0 Plasmid Maxiprep Kit _ 100

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  Price : 1110   EUR
1260   USD
861   GBP
4664   Zloty
148579   JPY
8563   NOK
9175   SEK
1255   CHF

Product name : JETSTAR 2.0 Plasmid Maxiprep Kit _ 100

Catalog number : 220 100

Quantity: 1

Availability: Yes

Supplier name : Genomed

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More Details about

JETSTAR Plasmid Purification Kit
 
  Mini column Midi column Maxi column
 
 
  Mega cartridge Giga cartridge  
 
JETSTAR specifications:
 
Product is designed to prepare plasmid DNA (small, medium and large scale) from E.coli cells
Product contents are gravity fast flow columns or vacuum cartridges, RNAse A, ready-to-use buffers and protocol
JETSTAR preparations are available in Mini, Midi, Maxi, Mega and Giga format
JETSTAR is able to prepare cosmid DNA and BAC DNA as well
No chromosomal DNA contamination
No phenol/chloroform extraction
No organic or toxic reagents
Excellent reproducibility
Total procedure is 40 - 60min
Plasmid DNA is ultrapure (better than 2 x CsCl)
Plasmid DNA recovery is 90% - 95% on average
Plasmid DNA of all sizes can be prepared
Plasmid DNA yields are 20µg (Mini), 100µg (Midi), 500µg (Maxi), 2.500µg (Mega), 10.000µg (Giga) in minimum
A260/280 ratio is > 1.85
Endotoxin level is < 0.01 EU/µg plasmid DNA
 
Applications for plasmid and cosmid DNA
 
Transfection
Manual and automated sequencing, including capillary sequencing
Microinjection
In vitro transcription
Restriction analysis, ligation, labeling etc.
Transfection of endotoxin-sensitive cells (Endo Free)
Gene therapy applications
 
 
 
 
Gel analysis (1% agarose gel) of a 3 ml high copy plasmid DNA (pBluescript II KS) preparation before (lane 1) and after plasmid purification with the JETSTAR 2.0 Plasmid Kit (lane 5). The RNA is found in the flow-through (lane two) and the washes (lanes 3 and 4).   Plasmid DNA (pBluescript II KS) was prepared using the JETSTAR 2.0 Plasmid Kit. The plasmid DNA was digested with EcoRI and ligated with a 1.65 kb fragment (lane 2 shows the DNAs before ligation). Ligations were set up for 1 hour at 25°C with 0.02 U (lane 3), 0.05 U (lane 4) and 0.1 U (lane 5) of T4 DNA ligase. Length standard in lane 1 is the Gibco BRL 1 kb ladder.

 

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