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Index / Genomed / JETFLEX Genomic DNA Purification Kit / Product Detail : 600 500 JETFLEX Genomic DNA Purification Kit
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Jul
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#600 500 JETFLEX Genomic DNA Purification Kit

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  Price : 315   EUR
358   USD
244   GBP
1325   Zloty
42226   JPY
2433   NOK
2607   SEK
356   CHF

Product name : JETFLEX Genomic DNA Purification Kit

Catalog number : 600 500

Quantity: 1

Availability: Yes

Supplier name : Genomed

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More Details about

 

Introduction:
The JetFlex Genomic DNA Purification Kits allow for the rapid and efficient purification of high quality, high molecular weight, RNA-free genomic DNA (gDNA). The kits are designed to efficiently isolate genomic DNA from mammalian cells and tissues, blood samples, plant tissue,bacteria, and yeast in a single tube without the use of spin columns or a vacuum manifold. The procedure is readily scaled up or down, making it adaptable for a wide variety of starting sample volumes. DNA up to 150 kb in size may be purified.
System summary:
Genomic DNA is purified with the JetFlex gDNA Purification Kits by lysing nuclei with a cell lysis buffer and if necessary,treating the sample with RNase A and Proteinase K to degrade RNA and proteins. DNA is then precipitated under high salt conditions to remove impurities. Following ethanol removal,
the DNA is redissolved in the DNA Dissolving Buffer (TE).
Advantages:
The advantages of using JetFlex Genomic DNA Purification Kits are:
• Efficient purification of genomic DNA up to 150 kb from a variety of samples such as mammalian cells and tissues,blood samples, body fluids, plant tissue, bacteria, and yeast
• Rapid purification of high-quality DNA within 30 minutes without using any organic or toxic reagents
• Minimal contamination from RNA
• Reliable performance of the purified DNA in PCR,restriction enzyme digestion, and Southern blotting

General Information:

 

Follow the recommendations below to obtain the best results:
• Maintain a sterile environment when handling DNA to avoid any contamination from DNases and ensure that no DNases are introduced into the sterile solutions of the kit
• Make sure all equipment that comes in contact with DNA is sterile, including pipette tips and microcentrifuge tubes
• To minimize DNA degradation, perform lysate preparation steps quickly, and avoid repeated freezing
and thawing of DNA samples
• Perform all centrifugation steps at room temperature
• Incubate sample overnight with DNA Dissolving Buffer
Safety information:
Follow the safety guidelines below when using the kit.
• Treat all reagents supplied in the kit as potential irritants.
• Wear a suitable lab coat, disposable gloves, and goggles.
• Handle all blood and tissue samples in compliance with established institutional guidelines and take the appropriate precautions (wear a laboratory coat, disposable gloves, and eye protection). Since afety
requirements for use and handling of blood and tissue samples may vary at individual institutions, consult the health and safety guidelines and/or officers at your institution.
• When processing blood and tissue samples, dispose of the waste appropriately as biohazardous waste.

 

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