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Index / Trevigen / Cultrex / Product Detail : 3415-001-03 Cultrex
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#3415-001-03 Cultrex

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  Price : 319   EUR
363   USD
248   GBP
1343   Zloty
42798   JPY
2466   NOK
2643   SEK
361   CHF

Product name : Cultrex

Catalog number : 3415-001-03

Quantity: 1 mg/ 1ml

Availability: Yes

Supplier name : Trevigen

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Cultrex® Stem Cell Qualified Human BME, *PathClear®
Catalog #: 3415-001-03

Cultrex® Stem Cell Qualified Human BME, *PathClear®


Description:

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound repair. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells. Cultrex® Human Basement Membrane Extract (BME) is a soluble form of basement membrane purified from human placenta. BME can be used for promotion and maintenance of an undifferentiated phenotype, or for the directed differentiation of precursor cells, under a variety of cell culture conditions, including stem cells, primary epithelial cells, endothelial cells and smooth muscle cells.

Specifications:

Concentration:

1 mg/ml

Source:

Human Placenta

Storage Buffer:

Dulbecco’s Modified Eagle’s medium containing 10 μg/ml gentamicin sulfate and no phenol red.

Storage/Stability:

Product is stable for a minimum of 3 months from date of shipment when stored at –80 °C.
Repeated freeze-thaws will destroy product integrity.

Materials Qualification:

Functional Assay:

  • Promotes the attachment of H9 human embryonic stem cells.
  • Effectively maintains human embryonic stem cells in a pluripotent state as evidenced by intracellular staining for the stem cell markers Oct-4 and Nanog.

*Sterility Testing:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility testing protocols.
  • No mycoplasma contamination detected by PCR.
  • Stem Cell Qualified Human BME, PathClear® is tested negative by PCR for different human pathogenic viruses including EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, VZV.
  • Endotoxin concentration < 20 EU/ml by LAL assay.

Safety Statement: Cultrex® Human BME, PathClear® contains human source material and
therefore should be treated as potentially infectious and handled at Biological Safety Level 2 to
minimize exposure.

 

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