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Product name : Cultrex
Catalog number : 3434-005-02
Quantity: 5 ml
Supplier name : Trevigen
Data sheet: Ask more or other datasheet now !
More Details about
Cultrex® Stem Cell Qualified Basement Membrane Extract (BME), PathClear®
Cultrex® Stem Cell Qualified Basement Membrane Extract (BME), PathClear® is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37 °C to form a reconstituted basement membrane. It is mainly comprised of laminin,collagen IV, entactin, and heparin sulfate proteoglycan. Cultrex® Stem Cell Qualified BME has been shown to provide an effective feeder-free surface for the attachment of and maintenance of human embryonic stem cells in a pluripotent state, thereby enabling its use for growth promotion or for study of stem cell differentiation. The PathClear® designation means that in addition to standard sterility, endotoxin and MAP testing, the BME is tested by PCR and is clear of 31 pathogens and viruses, including LDEV. Each lot is rigorously qualified in biological performance assays.
CONCENTRAT I O N :
Murine Engelbreth-Holm-Swarm (EHS) tumor.
Dulbecco’s Modified Eagle’s medium containing 10 μg/ml gentamycin sulfate, without
Product is stable for a minimum of 3 months from date of shipment when stored at –20 °C
in a manual defrost freezer. For optimal stability, store at –80 °C in aliquots; repeated
freeze–thaws will destroy product integrity.
• Promotes the attachment of H9 human embryonic stem cells.
• Effectively maintains human embryonic stem cells in a pluripotent state as evidenced by
intracellular stains for the stem cell markers Oct-4 and Nanog.
STERILITY TESTING :
- No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test.
- Negative by PCR test for: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
- Endotoxin concentrations ≤ 8 EU/ml by LAL assay
- 1. Angel, M. and M. F. Yanik. 2010. Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins. PLoS ONE. 5(7):e11756.
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