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Index / Trevigen / Anti-PAR Monoclonal Antibody w_Control / Product Detail : 4335-MC-100-AC Anti-PAR Monoclonal Antibody w_Control
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Sep
27th

#4335-MC-100-AC Anti-PAR Monoclonal Antibody w_Control

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  Price : 370   EUR
420   USD
287   GBP
1554   Zloty
49526   JPY
2854   NOK
3058   SEK
418   CHF

Product name : Anti-PAR Monoclonal Antibody w_Control

Catalog number : 4335-MC-100-AC

Quantity: 100

Availability: Yes

Supplier name : Trevigen

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About this Product :

Anti-PAR Monoclonal Antibody w_Control http://biotech-map.com/aa_search/table_sample.html?q=clone&filter=anti
Storage and handling
Antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.

More Details about

Description: Trevigen’s monoclonal antibody (cat# 4335-MC-100) is specific for poly(ADPribose) (PAR) polymer. It can be used to detect ribosylated proteins by ELISA, Western blot, immunocytochemistry in situ, and for immunopurification. It is supplied with a positive control for ELISA and Western blot consisting of a poly-ADP-ribosylated PARP-1 protein (PARP-PAR).
Storage Conditions: Store the anti-PAR monoclonal at -20°C (manual defrost freezer). Store the PARP-treated protein control at -80°C.
Physical State: This antibody is provided as purified immunoglobulin from mouse ascites in 1X PBS containing 50% glycerol at 1 mg/ml. PARP-PAR is provided at 75 μM in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Immunogen: Purified ADP-ribose polymers between 2 and 50 units long
Ig Class: IgG3
Specificity: The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers.
Applications: ELISA, Western analysis, immunoprecipitation, and immunopurification. For Western blots, an antibody dilution of 1:1000 is recommended.

Procedure for Immunoblotting using Peroxidase Detection:
Blotting buffer: 12 mM Tris base, 96 mM Glycine, and 15% MeOH.
Blocking solution: 5% (w/v) nonfat dry milk in TBS-0.1% Tween.
Antibody solution: 5% (w/v) nonfat dry milk, in TBS-0.1% Tween.


Transfer the electrophoresed proteins to an Immobilon-FL or PVDF membrane and incubate the membrane for 1 hour at room temperature in blocking solution.
Incubate the membrane overnight at 4°C in antibody solution containing a 1:1000 dilution of Trevigen’s anti-PAR monoclonal antibody. Empirical determination of the optimal monoclonal antibody concentration will be required for optimal results.


Wash the membrane at room temperature for 5 minutes with 4 changes of TBS-0.1% Tween. Changing the membrane containers often reduces background.
Incubate the membrane at room temperature for 1 hour in antibody solution containing antirabbit conjugated to horseradish peroxidase, or IR800-conjugated secondary antibody (Licor).
Empirical determination of secondary antibody concentration will be required for optimal results.
Wash the membrane for 15 minutes with 4 changes of TBS-0.1% Tween.
Develop peroxidase reaction using chemiluminescence, or as described in the figure 2
legend.

 

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