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Index / Ray Biotech / Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2 / Product Detail : 228-10099-2 Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2
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Sep
27th

#228-10099-2 Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2

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  Price : 354   EUR
401   USD
274   GBP
1487   Zloty
47379   JPY
2730   NOK
2926   SEK
400   CHF

Product name : Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2

Catalog number : 228-10099-2

Quantity: 10

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2 Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Recombinant Human B-Cell Leukemia _ Lymphoma 2 -BH2.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

 

Target Name

Bcl-2 -BH2

Target Species

Human

Application Notes

Additional Application Information
Input marker or positive control (Western blotting).
Function study.
Recommended Applications
Western blot;
Reconstitution
Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C. 
Dilute 10 fold into selected buffer system.
BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Recommended Applications
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Reconstitution
P10415

Storage/Stability

Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below 
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Format

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

Preparation

Bcl-2 Des BH2 domain (187-202 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 202 amino acids 1-186 and 203-218.
The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Expression System
Escherichia Coli.

Purification

Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

More Information

Target Synonyms
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Uniprot Accession Number
P10415
 
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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