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Index / Ray Biotech / Recombinant Rat Calbindin D-28K / Product Detail : 228-10155-3 Recombinant Rat Calbindin D-28K
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Aug
26th

#228-10155-3 Recombinant Rat Calbindin D-28K

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  Price : 3752   EUR
4258   USD
2910   GBP
15760   Zloty
501991   JPY
28932   NOK
31001   SEK
4243   CHF

Product name : Recombinant Rat Calbindin D-28K

Catalog number : 228-10155-3

Quantity: 100

Availability: Yes

Supplier name : Ray Biotech

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More Details about

 

Target Name

CaBP28K

Target Species

Rat

Application Notes

Additional Application Information
The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:A- Dilute 1 µl of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.D- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
Recommended Applications
Dot Blot; Immunohistochemistry;
Reconstitution
It is recommended to reconstitute the lyophilized CABP28K in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Recommended Applications
Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.
Reconstitution
P07171

Storage/Stability

Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Format

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3.

Preparation

Recombinant Rat Calbindin-1 produced in E.Coli.
The Rat CALB1 is purified by proprietary chromatographic techniques.
Expression System
Escherichia Coli.

Purification

Purity:
Greater than 90% as determined by SDS-PAGE.

More Information

Target Synonyms
Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.
Uniprot Accession Number
P07171
 
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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