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Index / Ray Biotech / Recombinant Thermostable dUTPase / Product Detail : 228-10346-3 Recombinant Thermostable dUTPase
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Sep
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#228-10346-3 Recombinant Thermostable dUTPase

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  Price : 1397   EUR
1586   USD
1083   GBP
5869   Zloty
186940   JPY
10774   NOK
11545   SEK
1580   CHF

Product name : Recombinant Thermostable dUTPase

Catalog number : 228-10346-3

Quantity: 2000 IU

Availability: Yes

Supplier name : Ray Biotech

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More Details about

 

Target Name

dUTPase

Target Species

Bacterial

Application Notes

Specific Activity
103u/ug.
Definition of Unit Activity
One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
Recommended Applications
Thermostable dUTPase, dUTPase.

Format

Physical Appearance
Sterile filtered liquid formulation.
Formulation
dUTPase is supplied in 20mM Tris-HC1 (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at concentration of 10 u/ul of the enzyme.

Preparation

Thermostable dUTPase (pyrococcus fruriosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall and slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications such as cloning and subsequent recombinant protein technology, and gene expression analysis (semi-quantitative RT-PCR techniques and real-time PCR analysis), where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair. dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis.
Expression System
Escherichia Coli.

Purification

Purity:
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

More Information

Target Synonyms
Thermostable dUTPase, dUTPase.
 
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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