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Index / Ray Biotech / Human Fibronectin / Product Detail : 228-10473-1 Human Fibronectin
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Aug
26th

#228-10473-1 Human Fibronectin

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  Price : 245   EUR
278   USD
190   GBP
1029   Zloty
32779   JPY
1889   NOK
2024   SEK
277   CHF

Product name : Human Fibronectin

Catalog number : 228-10473-1

Quantity: 200

Availability: Yes

Supplier name : Ray Biotech

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More Details about

 

Target Name

Fibronectin

Target Species

Human

Application Notes

Additional Application Information
Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with a suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5ug/cm² of growth surface.
Recommended Applications
Cell & Tissue Culture;
Reconstitution
Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. 
Let stand 1-2 hours.

Storage/Stability

Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.

Format

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl.

Preparation

Native Protein Source
Human Plasma.

Purification

Purity:
Greater than 95.0% as determined by SDS-PAGE.
 
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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