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Index / Bioassays / QuantiChrom™ BCG Albumin Assay Kit / Product Detail : DIAG-250 QuantiChrom™ BCG Albumin Assay Kit
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Dec
8th

#DIAG-250 QuantiChrom™ BCG Albumin Assay Kit

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  Price : 363   EUR
412   USD
282   GBP
1527   Zloty
48667   JPY
2805   NOK
3005   SEK
411   CHF

Product name : QuantiChrom™ BCG Albumin Assay Kit

Catalog number : DIAG-250

Quantity: 250

Availability: Yes

Supplier name : Bioassays

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About this Product :

QuantiChrom™ BCG Albumin Assay Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of QuantiChrom™ BCG Albumin Assay Kit.
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DESCRIPTION
Albumin is the most abundant plasma protein in human. It accounts for
about 60% of the total serum protein. Albumin plays important
physiological roles, including maintenance of colloid osmotic pressure,
binding of key substances such as long-chain fatty acids, bile acids,
bilirubin, haematin, calcium and magnesium. It has anti-oxidant and
anticoagulant effects, and also acts as a carrier for nutritional factors and
drugs, as an effective plasma pH buffer. Serum albumin is a reliable
prognostic indicator for morbidity and mortality, liver disease, nephritic
syndrome, malnutrition and protein-losing enteropathies. High levels are
associated with dehydration.
Simple, direct and automation-ready procedures for measuring albumin
concentration in biological samples are becoming popular in Research
and Drug Discovery. BioAssay Systems' BCG albumin assay kit is
designed to measure albumin directly in biological samples without any
pretreatment. The improved method utilizes bromcresol green that forms
a colored complex specifically with albumin. The intensity of the color,
measured at 620nm, is directly proportional to the albumin concentration
in the sample. The optimized formulation substantially reduces
interference by substances in the raw samples.
KEY FEATURES
Sensitive and accurate. Use as little as 5 μL samples. Detection range
0.01 g/dL (1.5μM) to 5 g/dL (750μM) albumin in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a
single working reagent and incubation for 5 min. Can be readily
automated as a high-throughput assay in 96-well plates for thousands of
samples per day.
Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
No interference in biological samples. No pretreatments are needed.
Assays can be directly performed on raw biological samples i.e., in the
presence of lipid and protein.
APPLICATIONS:
Direct assays: albumin in serum, plasma, urine, biological preparations.
Drug discovery/Pharmacology: effects of drugs on albumin metabolism.
KIT CONTENTS (250 tests in 96-well plates)
Reagent: 50 mL Albumin Standard: 1 mL 5 g/dL BSA
Storage conditions. The kit is shipped at room temperature. Store
Reagent and standard at 4°C and -20°C, respectively. Shelf life of 12
months after receipt.
Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.
PROCEDURES
Reagent Preparation:
Important: bring reagent to room temperature and shake before use.
Procedure using 96-well plate:
1. Dilute standards in distilled water as follows. Dilute serum and plasma
samples 2 fold. Transfer 5 μL diluted standards and diluted samples to
wells of a clear bottom plate. Store diluted standards at -20°C for
future use.
No STD + H2O Vol (μL) BSA (g/dL)
1 100μL + 0μL 100 5.0
2 80μL + 20μL 100 4.0
3 60μL + 40μL 100 3.0
4 40μL + 60μL 100 2.0
5 30μL + 70μL 100 1.5
6 20μL + 80μL 100 1.0
7 10μL + 90μL 100 0.5
8 0μL + 100μL 100 0
2. Add 200 μL working reagent and tap lightly to mix. Avoid bubble.
3. Incubate 5 min at room temperature and read optical density at 570-
670nm (peak absorbance at 620nm).
Procedure using cuvette:
1. Transfer 20 μL Blank, Standards and samples to appropriately
labeled tubes. Add 1000 μL working reagent and tap lightly to
mix. Incubate 5 min at room temperature.
2. Transfer to cuvet and read optical density at 620nm.
Important: if sample OD is higher than the OD for standard, dilute
samples with distilled water and repeat the assay.
CALCULATION
Subtract blank OD (water, #8) from the standard OD values and plot
the OD against standard concentrations. Use the standard curve to
determine the sample albumin concentration.
Conversions: 0.1 g/dL albumin equals 15 μM, 0.1% or 1000 ppm.
MATERIALS REQUIRED, BUT NOT PROVIDED
Pipeting devices and accessories (e.g. 5 μL).
Procedure using 96-well plate:
Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.
Procedure using cuvette:
Spectrophotometer and cuvets for measuring OD at 620nm.
 

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