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#EKIN-400 EnzyChrom™ Kinase Assay Kit
Product name : EnzyChrom™ Kinase Assay Kit
Catalog number : EKIN-400
Supplier name : Bioassays
Data sheet: Ask more or other datasheet now !
More Details aboutThe EnzyChrom™ Kinase Assay Kit is Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors.Safe. Non-radioactive assay.Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature.Robust and amenable to HTS: Assay can tolerate up to 300 μM ATP and 10% dimethylsulfoxide (DMSO). Z’ factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day.Kinases , also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments. The EnzyChrom™ Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay method.#2#http://biotech-map.com/aa_search/table_sample.html?q=EnzyChrom%E2%84%A2+Kinase+Assay+Kit&filter=
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WP1004: Kit Receptor Signaling Pathway
WP1121: Kit Receptor Signaling Pathway
WP1341: Kit Receptor Signaling Pathway
WP147: Kit Receptor Signaling Pathway
WP1493: Carbon assimilation C4 pathway
WP1567: Glycolysis and Gluconeogenesis
WP1619: Amino sugar and nucleotide sugar metabolism
WP1653: Galactose metabolism
WP1681: Pantothenate and CoA biosynthesis
WP1701: Starch and sucrose metabolism
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WP1844: MAP kinase cascade
WP1946: Cori Cycle
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP32: Translation Factors
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WP548: neural crest development
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