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#K008 Mycoplasma agalactiae
Product name : Mycoplasma agalactiae
Catalog number : K008
Supplier name : GeneRiver
Data sheet: Ask more or other datasheet now !
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Technical details for ready to use PCR kit Mycoplasma agalactiae (gel agarose based)
K008 Mycoplasma agalactiae, 100 reactions
Mycoplasma agalactiae is a pathogenic mycoplasma causing contagious agalactia in small ruminants like sheep. This shows acute as well as subacute clinical symptoms like masitis, keratitis, arthritis etc., therefore there is a need of laboratory diagnosis. This laboratory diagnosis can be done through culture methods (which takes a longtime as Mycoplasma goes slowly and needs special media), serological methods like ELISA as well as FAT, which are prone to cross reactions, therefore the PCR is merging as a goldstandard for detection of micro organisms in medicine as this is highly sensitive and specific also. Moreover PCR needs a little material to conduct the test.
Target gene: highly conserved region of gene of Mycoplasma agalactiae
Kinds of samples: milk, colonies grown on cultures, blood (serum/plasma) samples, tissue samples and other body fluid samples. This kit needs isolated DNA, which can be isolated using commercial available kits or home made methods.
Components of the kit: polymerase, forward and backward primers, nucleotides, buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control, negative control as well as manual.
Validations: It has been tested for cross reactions with related and non related micro organisms like Mycoplasma bovis, Mycoplasma mycoides, different Staphylocci, Staphylococcus aereus, different Streptococci, E.coli and many more . It is tested for serial dilutions.
Sensitivity: 2.5-25 fg of Mcoplasma agalactiae
Only for in vitro use
Only for veterinary use
to be used through technical person
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[MPN_348 H91_orf164 MP488] 5-formyltetrahydrofolate cyclo-ligase (EC 126.96.36.199) (5,10-methenyl-tetrahydrofolate synthetase) (MTHFS) (Methenyl-THF synthetase)
[MG335.2] Processive diacylglycerol beta-glycosyltransferase (EC 2.4.1.-) (Beta-diglycosyldiacylglycerol synthase) (Beta-DGS) (DGlyDAG synthase) (Beta-monoglycosyldiacylglycerol synthase) (Beta-MGS) (MGlyDAG synthase) (Glycosyl-beta-1,6-glycosyldiacylglycerol synthase) (UDP-galactose:1,2-dioleoylglycerol 3-beta-D-galactosyltransferase) (UDP-glucose:1,2-dioleoylglycerol 3-beta-D-glucosyltransferase)
[ribF/mnmA MYCGA1200 MGA_0832] Trifunctional protein RibF/MnmA [Includes: FMN adenylyltransferase (EC 188.8.131.52) (FAD pyrophosphorylase) (FAD synthase); Riboflavin kinase (EC 184.108.40.206) (Flavokinase); tRNA-specific 2-thiouridylase MnmA (EC 220.127.116.11)]
[nrnA MPN_140 MP014] Bifunctional oligoribonuclease and PAP phosphatase NrnA (EC 3.1.-.-) (3'(2'),5'-bisphosphate nucleotidase) (EC 18.104.22.168) (3'-phosphoadenosine 5'-phosphate phosphatase) (PAP phosphatase) (Mgp-operon protein 1) (Mgp1) (ORF-1 protein) (nanoRNase)
[ptsH MCAP_0716] Phosphocarrier protein HPr (EC 2.7.11.-) (Histidine-containing protein)
[pyrG ctrA MSC_0134] CTP synthase (EC 22.214.171.124) (Cytidine 5'-triphosphate synthase) (Cytidine triphosphate synthetase) (CTP synthetase) (CTPS) (UTP--ammonia ligase)
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[hprK ptsK MSC_0940] HPr kinase/phosphorylase (HPrK/P) (EC 2.7.11.-) (EC 2.7.4.-) (HPr(Ser) kinase/phosphorylase)
[pfk pfkA MSC_0260] ATP-dependent 6-phosphofructokinase (ATP-PFK) (Phosphofructokinase) (EC 188.8.131.52) (Phosphohexokinase)
[ptsI MCAP_0233] Phosphoenolpyruvate-protein phosphotransferase (EC 184.108.40.206) (Phosphotransferase system, enzyme I)
[ackA MSC_0270] Acetate kinase (EC 220.127.116.11) (Acetokinase)
[eno MSC_0253] Enolase (EC 18.104.22.168) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)
[metK MSC_0492] S-adenosylmethionine synthase (AdoMet synthase) (EC 22.214.171.124) (MAT) (Methionine adenosyltransferase)
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[pyrG MLC_1210] CTP synthase (EC 188.8.131.52) (Cytidine 5'-triphosphate synthase) (Cytidine triphosphate synthetase) (CTP synthetase) (CTPS) (UTP--ammonia ligase)
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[hlp3 hmw3 MYCGA1770 MGA_0928] Cytadherence high molecular weight protein 3 (Accessory adhesin protein 3) (Cytadherence accessory protein 3)
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[folD MSC_0758] Bifunctional protein FolD [Includes: Methylenetetrahydrofolate dehydrogenase (EC 126.96.36.199); Methenyltetrahydrofolate cyclohydrolase (EC 188.8.131.52)]
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