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#LM-D1220/500 DMEM _ F12 w_o L_ Glutamine w_o Hepes _ 500ml
Product name : DMEM _ F12 w_o L_ Glutamine w_o Hepes _ 500ml
Catalog number : LM-D1220/500
Supplier name : Biosera
Data sheet: Ask more or other datasheet now !
More Details about
Product Code: LM-D1220
Description: DMEM Ham’s F12 w/o L-Glutamine, w/o Hepes Theoretical
pH: 7.4 ± 0.3
Osmolarity: 334 mOsm/kg 10%
Colour: Red solution
Storage conditions: +4C in the dark
Shelf life: 24 months
Endotoxin: <1 EU/ml
Composition: Available on request
- bacteria aerobic-anaerobic
- bacteria, strictly anaerobic
Composition Available upon request Cell Growth Test Medium tested for the ability to support cell growth with Hela line.
Use aseptic technique when handling or supplementing this medium. Product is provided for laboratory use only, and not for drug, human or veterinary use. Description/Application Studies to determine the nutritional requirements of many cells have been in progress since Eagle’s first reports. The major essential nutrients were identified and work became focused on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM medium and Ham’s F12 medium, known as DMEM Ham’s F12. Uses Supplements, such as antibiotics, should be added as sterile supplements to the medium. Storage conditions and shelf-life of supplemented products will be affected by the nature of the supplements. Add 12.5 ml/l of L-Glutamine 100X, 200mM (cat. : XC-T1715) or 365 mg/l of L-Glutamine (cat. : PM-P1410) before using this medium.
Indication of Deterioration:
Medium should be clear and free of particulate and flocculent material. Do not use this medium if it is cloudy or contains precipitate. Other evidence of deterioration may include colour change or degradation of physical or performance characteristics.
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WP1643: D-Glutamine and D-glutamate metabolism
WP1718: Vitamin B6 metabolism
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