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#LM-A4116/100 Nanomycopulitine Concentrat 20 x _ 100ml
Product name : Nanomycopulitine Concentrat 20 x _ 100ml
Catalog number : LM-A4116/100
Supplier name : Biosera
Data sheet: Ask more or other datasheet now !
More Details about
1. Intended use
Continuous use of sterile cell culture equipment, media and cells, leads to long-term microbial contamination risk. A number of research and industrial applications involve antibiotics or toxins to reduce this risk. Applications where non-sterile cells were taken into cell culture are dependent of supplements suppressing contaminants. There are also circumstances where physical contaminant removal is inappropriate because filtration compromises the component viability or activity.
The toxin includes different required advantages for a suitable application in routine cell culture:
-The NanomycopulitineR preferably eliminates bacteria.
-It is active and kills bacteria in both, logarithmic and stationary growing life cycle phases.
-The toxin does not affect the normal metabolism or enzymatic function.
-The solutions do not contain excipients or buffer which influence the growth negatively.
-The toxin is stable in liquid form under normal storage conditions and also in cell culture to provide protection over a period of time
-NanomycopulitineR has a wide spectrum of activity against gram negative and gram positive bacteria nd also others like Chlamydia,
Mycobacteria, a broad range of Mycoplasma, Nanobacteria and bacteria L-forms.
-It is non toxic and safe with respect to human handling
NanomycopulitineR can be used in vitro to clean already contaminated cell cultures and to prevent recontamination during further cultivation.
Curing of Mycoplasma infected cell lines is one of the most important applications of this innovative agent. Another useful
NanomycopulitineR application is media protection against contamination if sterile filtration is not appropriate.
-This test is designed for 1 ml concentrated NanomycopulitineR 20X concentrated solution
Use a 24 well plate
To prepare the NanomycopulitineR with a 1/20 as dilution in your regular cell culture medium ( It could be 1/15, 1/25 or 1/30
depending on the cell type)
-Resuspend 5 x 104 cells in 1 ml of the selected dilution
-Seed the cells and grow them for several days
-Check growth and morphology to compare them with your control culture
Notice: In between Nanomycopulitinal treatments, there should be cultivation without the addition of NanomycopulitineR to devoid resistant bacterial forms.
Filter sterilized by 0.2 £gm
5. Solution Stability
Stable at 4¢XC for 2 weeks and 24 months as concentrate at ¡V20¢XC
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