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Index / Biovis / Annexin V_Cy3 Reagent200 assays / Product Detail : 1002-200 Annexin V_Cy3 Reagent200 assays
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Aug
28th

#1002-200 Annexin V_Cy3 Reagent200 assays

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  Price : 395   EUR
449   USD
307   GBP
1662   Zloty
52961   JPY
3052   NOK
3270   SEK
447   CHF

Product name : Annexin V_Cy3 Reagent200 assays

Catalog number : 1002-200

Quantity: 200 assays

Availability: Yes

Supplier name : Biovis

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More Details about

DESCRIPTION:
The bright red color fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. The Annexin V-Cy3 conjugate contains the bright red fluorescent probe that can be used for detection of apoptosis by fluorescence microscopy with a rhodamine filter or by flow cytometry. Cy3 yields red fluorescence with a max emission of 570 nm.

Product Highlights:
•Detection method- flow cytometry (Ex/Em- 543/570 nm) using FL2 channel and fluorescence microscopy
•Sample- Cell culture
•Simple, easy to use and convenient

Storage Temp.: +4°C

Shipping: gel pack

Usage: For Research Use Only! Not For Use in Humans.


ASSAY PROTOCOL:
A. Incubation of cells with Annexin V-Cy3:
1. Induce apoptosis by desired methods.
2. Collect 1 x 105 cells by centrifugation.
3. Resuspend cells in 500 μl of 1X Annexin V Binding Buffer (Cat.#1035-100).
4. Add 1 μl of Annexin V-Cy3.
5. Incubate at room temperature for 5 min in the dark.
Proceed to B or C below depending on method of analysis.
B. Quantification by Flow Cytometry:
Analyze cells by flow cytometry (Ex = 543 nm; Em = 570 nm) using FL2 channel. For adherent cells, trypsinize and gently wash cells with serum-containing medium before incubation with Annexin V-Cy3 (A.3-5).
C. Detection by Fluorescence Microscopy:
1. Place the cell suspension from Step A.5 on a glass slide, and cover with a glass coverslip. For analyzing adherent cells, grow cells directly on a coverslip. Following incubation (A.5), invert coverslip on a glass slide and visualize cells. The cells can also be washed with 1X Annexin V Binding Buffer and fixed in 2% formaldehyde before visualization.
(Cells must be incubated with Annexin V-Cy3 before fixation because any cell membrane disruption can cause nonspecific binding of annexin V to PS on the inner surface of the cell membrane.)
2. Observe the cells under a fluorescence microscope using a rhodamine filter.
Cells that have bound Annexin V-Cy3 will show bright red staining on the plasma membrane.

 

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