GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Biovis / Annexin V_EGFP Reagent200 assays / Product Detail : 1004-200 Annexin V_EGFP Reagent200 assays
Related keywords:

Sep
28th

#1004-200 Annexin V_EGFP Reagent200 assays

Ask technical file .

  Price : 395   EUR
449   USD
307   GBP
1662   Zloty
52961   JPY
3052   NOK
3270   SEK
447   CHF

Product name : Annexin V_EGFP Reagent200 assays

Catalog number : 1004-200

Quantity: 200 assays

Availability: Yes

Supplier name : Biovis

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

STORAGE COITIONS: Store at 4°C (short term), -20°C (long term).
SHELF LIFE: 1 year under proper storage conditions
Shipping: gel pack

Product Highlights:
•Detection method- flow cytometry (Ex/ Em- 488 /530 nm) using FITC signal detector (usually FL1) and fluorescence microscopy
•Sample- Cell culture
•Simple, easy to use and convenient

Usage: For Research Use Only! Not For Use in Humans.


DESCRIPTION:
The bright and photo-stable reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. The annexin V-EGFP fusion is brighter and photo-stable, ideal for detecting apoptosis by fluorescence microscopy with a FITC filter or by flow cytometry.


ASSAY PROTOCOL:
A. Incubation of cells with Annexin V-EGFP
1. Induce apoptosis by desired method.
2. Collect 1-5 x 105 cells by centrifugation.
3. Resuspend cells in 500 μl of 1X Binding Buffer (Cat.# 1035-100).
4. Add 1 μl of Annexin V-EGFP and 1 μl of propidium iodide (PI, Cat.# 1056-1)
5. Incubate at room temperature for 5 min in the dark.
Proceed to B or C below depending on method of analysis.
B. Quantification by Flow Cytometry
Analyze Annexin V-EGFP binding by flow cytometry (Ex = 488 nm; Em = 530 nm) using FITC signal detector (usually FL1) and PI staining by the phycoerythrin emission signal detector (usually FL2).
For adherent cells, gently trypsinize and wash cells once with serum-containing media before incubation with Annexin V-EGFP (A.3-5).
C. Detection by Fluorescence Microscopy
1. Place the cell suspension from Step A.5 on a glass slide. Cover the cells with a glass coverslip.
For analyzing adherent cells, grow cells directly on a coverslip. Following incubation (A.5), invert coverslip on glass slide and visualize cells. The cells can also be washed and fixed in 2% formaldehyde before visualization.
Note: Cells must be incubated with Annexin V-EGFP before fixation since any cell membrane disruption can cause nonspecific binding of Annexin V to PS on the inner surface of the cell membrane.
2. Observe the cells under a fluorescence microscope using a dual filter set for FITC & rhodamine. Cells which have bound Annexin V-EGFP will show green staining in the plasma membrane. Cells which have lost membrane integrity will show red staining (PI) throughout the nucleus and a halo of green staining (EGFP) on the cell surface (plasma membrane).

 

RELATED PRODUCTS:


Apoptosis Detection Kits & Reagents

  • Annexin V Kits & Bulk Reagents
  • Caspase Assay Kits & Reagents
  • Mitochondrial Apoptosis Kits & Reagents
  • Nuclear Apoptosis Kits & Reagents
  • Apoptosis Inducers and Set
  • Apoptosis siRNA Vectors

 

Cell Fractionation System

  • Mitochondria/Cytosol Fractionation Kit
  • Nuclear/Cytosol Fractionation Kit
  • Membrane Protein Extraction Kit
  • Cytosol/Particulate Rapid Separation Kit
  • Mammalian Cell Extraction Kit
  • FractionPREP Fractionation System

 

Cell Proliferation & Senescence

  • Quick Cell Proliferation Assay Kit
  • Senescence Detection Kit
  • High Throughput Apoptosis/Cell Viability Assay Kits
  • LDH-Cytotoxicity Assay Kit
  • Bioluminescence Cytotoxicity Assay Kit
  • Live/Dead Cell Staining Kit


Cell Damage & Repair

  • HDAC Fluorometric & Colorimetric Assays & Drug Discovery Kits
  • HAT Colorimetric Assay Kit & Reagents
  • DNA Damage Quantification Kit
  • Glutathione & Nitric Oxide Fluorometric & Colorimetric Assay Kits


Signal Transduction

  • cAMP & cGMP Assay Kits
  • Akt & JNK Activity Assay Kits
  • Beta-Secretase Activity Assay Kit


Adipocyte & Lipid Transfer

  • Recombinant Adiponectin, Survivin, & Leptin
  • CETP Activity Assay & Drug Discovery Kits
  • PLTP Activity Assay & Drug Discovery Kits
  • Total Cholesterol Quantification Kit


Molecular Biology & Reporter Assays

  • siRNA Vectors
  • Cloning Insert Quick Screening Kit
  • Mitochondrial & Genomic DNA Isolation Kits
  • 5 Minutes DNA Ligation Kit
  • 20 Minutes Gel Staining/Destaining Kit
  • β -Galactosidase Staining Kit & Luciferase Reporter Assay Kit


Growth Factors and Cytokines
Monoclonal and Polyclonal Antibodies

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Biovis \ Annexin_V_EGFP_Reagent200_assays \ 1004_200
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur