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Index / Biovis / Active Human Caspase_2100 units / Product Detail : 1082-100 Active Human Caspase_2100 units
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#1082-100 Active Human Caspase_2100 units

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  Price : 360   EUR
409   USD
279   GBP
1514   Zloty
48238   JPY
2780   NOK
2979   SEK
407   CHF

Product name : Active Human Caspase_2100 units

Catalog number : 1082-100

Quantity: 100 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Active Human Caspase_2100 units Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Active Human Caspase_2100 units.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
PURITY: >95% by SDS-PAGE


STORAGE CONDITIONS:
The lyophilized caspase-2 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.
The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA (Cat. #1072-200) or Ac-VDVAD-AFC (1071-200).


APPLICATIONS AND USAGE:
Active caspase-2 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to BioVision’s Caspase-2 Fluorometric or Colorimetric Assay Kits (Cat.#: K116 and K117).

 

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