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Index / Biovis / Active Human Caspase_10_a25 units / Product Detail : 1090A-25 Active Human Caspase_10_a25 units
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#1090A-25 Active Human Caspase_10_a25 units

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  Price : 222   EUR
252   USD
172   GBP
934   Zloty
29773   JPY
1716   NOK
1838   SEK
251   CHF

Product name : Active Human Caspase_10_a25 units

Catalog number : 1090A-25

Quantity: 25 units

Availability: Yes

Supplier name : Biovis

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About this Product :

Active Human Caspase_10_a25 units Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Active Human Caspase_10_a25 units.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

UNIT DEFINITION:
One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 8000 units/mg
PURITY: >90% by SDS-PAGE


STORAGE CONDITIONS:
The lyophilized caspase-10 is stable for 1 year at –70°C. Following reconstitution in PBS+15% glycerol, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).


APPLICATIONS AND USAGE:
Active caspase10/a is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase assay protocol, please refer to BioVision’s Caspase Fluorometric or Colorimetric Assay Kits.


For Research Use Only! Not to be used in humans.

 

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