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Index / Biovis / Active Cathepsin L100 ug / Product Detail : 1135-100 Active Cathepsin L100 ug
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Sep
27th

#1135-100 Active Cathepsin L100 ug

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  Price : 395   EUR
449   USD
307   GBP
1662   Zloty
52961   JPY
3052   NOK
3270   SEK
447   CHF

Product name : Active Cathepsin L100 ug

Catalog number : 1135-100

Quantity: 100 ug

Availability: Yes

Supplier name : Biovis

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More Details about

BACKGROUND DESCRIPTION:
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer.


SOURCE:
The recombinant Cathepsin L was produced from E. coli encoding human cDNA sequence from 188 to 1135 of gene accession number M20496 with 6XHis-tag on its N-terminus. The protein was purified by several chromatographic steps.


FORM: Lyophilized from 50 mM HEPES buffer, pH 4.0.
PURITY: >90% by SDS-PAGE
RECONSTITUTION: With dH2O to 0.1 – 1 mg/ml.


STORAGE CONDITIONS:
Store at –70°C, stable for at least 1 year as supplied. Avoid freeze/thaw cycles.


APPLICATIONS:
Recombinant Cathepsin L is useful in inhibitor screening, kinetic studies and cleavage of substrates in vitro or in tissue culture.


SPECIFIC ACTIVITY:
6000 units/mg protein. One unit will hydrolyze one nmol Z-Phe-Arg-AFC substrate per minute at 37°C in 400 mM Sodium Acetate, pH 5.5, with 4 mM EDTA and 10 mM DTT.

 

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