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Index / BPS Bioscience / PDE4A Assay Kit / Product Detail : 60340 PDE4A Assay Kit
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Jul
28th

#60340 PDE4A Assay Kit

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  Price : 614   EUR
697   USD
476   GBP
2579   Zloty
82162   JPY
4735   NOK
5074   SEK
694   CHF

Product name : PDE4A Assay Kit

Catalog number : 60340

Quantity:  100 reactions 

Availability: Yes

Supplier name : BPS Bioscience

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More Details about

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE4A1A is widely expressed in brain tumors
and promotes their growth and its inhibitor Rolipram is identified as a potent anti-inflammatory
agent. The PDE4A1A Assay Kit is designed for identification of PDE4A1A
inhibitors using fluorescence polarization. The assay is based on the binding of a
fluorescent nucleotide monophosphate generated by PDE4A1A to the binding agent.

Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled
cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide
product. This increases the size of the nucleotide relative to unreacted cyclic
monophosphate. In the polarization assay, dye molecules with absorption transition
vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes
attached to the rapidly-rotating cyclic monophosphates will obtain random orientations
and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead
complexes will not have time to reorient and therefore will emit highly polarized light.

The PDE4A1A inhibitor screening assay kit comes in a convenient 96-well format,
including purified PDE4A1A enzyme, fluorescently labeled PDE4A substrate (cAMP),
binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the
PDE4A1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on
a microtiter plate are required for PDE4A1A reactions. First, the fluorescently labeled
cAMP is incubated with a sample containing PDE4A1A for 1 hour. Second, a binding
agent is added to the reaction mix to produce a change in fluorescent polarization that
can then be measured using a fluorescence reader equipped for the measurement of
fluorescence polarization.

COMPONENTS:
PDE4A Assay Components

 

 

 

 

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: 6 months from date of receipt when stored as directed.

REFERENCE(S): Goldhoff P., et al. Clin Cancer Res. 2008; 14(23):7717-25.

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